对羟基苯乙腈水合酶高产菌株的选育  

Isolation of strains highly yielding p-hydroxyphenylacetamide nitrile hydratase

作　　者：蔡谦[1] 吴明火[1] 郑裕国[1] 沈寅初[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,杭州310032

出　　处：《生物加工过程》2005年第3期38-41,共4页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展规划项目(No.2003CB716005;973项目)

摘　　要：从全省各处采集的50多份土样筛选到一株产腈水合酶能力较高的菌株E10a,该菌株产生的腈水合酶为非诱导酶,产酶条件优化实验结果表明,产酶培养基组成:麦芽糖20 g/L,酵母膏5 g/L,尿素7.5 g/L,味精0.75 g/L,K2HPO40.5 g/L,KH2PO40.5 g/L,MgSO40.5 g/L,FeSO4.7H2O 10 mg/L,CoCl210 mg/L,微量元素母液0.8 ml/L;最佳培养条件为:培养温度28℃,摇床转速150 r/min,培养基起始pH 7.0,培养时间5 d,在优化培养条件下,1 h可将1 g/L质量浓度的底物对羟基苯乙腈全部转化为对羟基苯乙酰胺。A strain producing p-hydroxyphenylacetamide nitrile hydratase was isolated from above 50 soil samples collected from different regions of Zhejiang province. The medium for producing p-hydroxyphenylacetamide nitrile hydratases was composed of： malt sugar 20 g/L, yeast 5 g/L, urea 7.5 g/L, monosodium glutamate 0.75 g/L,K2HPO4 0.5 g/L,KH2PO4 0.5 g/L, MgSO4 0.5 g/L, FeSO4 ·7H2O 10 mg/L, CoCl2 10 mg/L, trace elements 0.8 mL/L. The optimal conditions were as follows： suitable initial pH value of culture medium was 7.0 at temperature 28 ℃, shaking at 150 r/min for 120 hours. The 1 g/L p-hydroxylacetonitrile could be totally transformed to p-hydroxyphenylacetaminde in 1 hour under optimal conditions.

关 键 词：对羟基苯乙酰胺(HPAD) 对羟基苯乙腈 腈水合酶 

分 类 号：Q933[生物学—微生物学]