ALG-SiO_2杂化凝胶固定化多酶体系催化CO_2转化甲醇研究  

Conversion of CO_(2) to methanol through multi-enzymes immobilized in ALG-SiO_(2) composite

作　　者：许松伟[1] 陆杨[1] 苑伟康[1] 吴洪[1] 姜忠义[1] 

机构地区：[1]天津大学化工学院,天津300072

出　　处：《生物加工过程》2005年第3期64-68,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(No.20176039)

摘　　要：采用甲酸脱氢酶(FateDH)、甲醛脱氢酶(FaldDH)和醇脱氢酶(ADH)3种脱氢酶为催化剂,以原型烟酰胺腺嘌呤二核甙酸(NADH)作为电子供体,通过3步连串反应将CO2转化为甲醇。采用正硅酸甲酯原位水解方法将二氧化硅掺杂于海藻酸(ALG)溶胶中,通过双交联制备出新型ALG-SiO2杂化凝胶。与ALG凝胶相比,ALG-SiO2结构更加紧密,酶的泄漏率大大降低,酶活性得以很好保持。将酶包埋于ALG-SiO2后,存放60 d以后或重复使用10次以上,酶活性都能保持80%以上,与之相比,包埋于ALG凝胶中的酶活性几乎完全丧失。Herein, CO2 was converted into methanol through three step reduction reactions catalyzed by different kinds of dehydrogenases, formate dehydrogenase （ FateDH）, formaldehyde dehydrogenase （FateDH） and alcohol dehydrogenase（ADH）. Reduced nicotinamide adenine dinucleotide （NADH） was employed as the elec- tron donor. Alginate-silica （ALG-SiO2） hybrid composite bead were prepared for the three dehydrogenases immobilization, through the hydrolysis and condensation of tetramethoxysilane （TMOS） in situ in an alginate solution, followed by Ca^2＋ crosslinking with blocks of guluronic acid residues. Compared to ALG gel, the ALG-SiO2 composite was compact, and feasible for enzyme loading and enzymatic activity retention. After the ALG-SiO2- enzyme biocomposite was stored for more than 60 days, or used for 10 cycles, more than 80% of the relative enzymatic activity could be retained. However, the relative enzymatic activity in the ALG-enzyme biocomposite was decreased almost to zero.

关 键 词：海藻酸 二氧化硅 杂化 固定化 二氧化碳 甲醇 

分 类 号：Q814[生物学—生物工程]