壳聚糖-DNA复合物形成机理研究  

Study on The Mechanism of Chitosan Complex Formation with PEGF-PC_3DNA

作　　者：陈郧东[1] 蔡妙颜[1] 张英[2] 郑文玲 马文丽 

机构地区：[1]华南理工大学,广东广州510640 [2]广州中医药大学,广东广州510405 [3]华南基因组中心,广东广州510515

出　　处：《药物生物技术》2005年第5期291-293,302,共4页Pharmaceutical Biotechnology

基　　金：国家自然科学基金项目(No.39880044)

摘　　要：为了探讨壳聚糖-DNA复合物的形成机理,通过向Na2SO4、DNA和溴化乙啶(EB)的混合溶液中分别加入不同体积的壳聚糖溶液,运用荧光分光光度计检测溶液荧光值的变化,发现随着壳聚糖浓度的增加,荧光值下降,说明壳聚糖和EB DNA之间的结合存在竞争性;通过测定Na2SO4离子浓度对壳聚糖-EB-DNA体系的影响发现,随着离子浓度的增加,壳聚糖-EB-DNA三者形成的平衡体系整体荧光强度不断减弱,这说明,壳聚糖是以静电与DNA相互作用的。To inquire into the mechanism DNA （pEGFPC3） complex＇ formation, different volume chitosan solution added to Na2SO4, DNA and EIA mixture. Experiment showed that fluorescent value was descending with the increasing of chitosan volume. It showed there was a plateau for the competitive binding of chitosan and ethidium bromide（EB） with DNA. It was also proved by the Na2SO4 ionic strength assay. Chitosan-EB-DNA balance system＇ fluorescent value reducing with raising Na2SO4 ionic concentration indicated chitosan interrected with DNA by static electricity.

关 键 词：壳聚糖 基因治疗 pEGFPC3质粒 DNA复合物 壳聚糖 机理研究 荧光分光光度计 离子浓度 形成机理 混合溶液 溴化乙啶 荧光强度 

分 类 号：R979.1[医药卫生—药品] R318.08[医药卫生—药学]