单纯疱疹病毒和载脂蛋白E基因多态性对阿尔茨海默病的影响  

The Influences of HSV Infection and the Single Nucleotide Polymorphism of Apolipoprotein E Gene on Alzheimer Disease

作　　者：龙燕 左丽[1] 李光荣[2] 裴华[1] 商正玲[1] 赵明祥[3] 

机构地区：[1]贵阳医学院免疫学教研室 [2]贵州省人民医院干部综合病房,贵州贵阳550002 [3]贵阳医学院附院神经科,贵州贵阳550004

出　　处：《贵阳医学院学报》2005年第5期392-395,共4页Journal of Guiyang Medical College

基　　金：贵州省优秀科技教育人才省长专项基金资助项目(1209)

摘　　要：目的:探讨单纯疱疹病毒感染和载脂蛋白(ApoE)基因多态性对阿尔茨海默病发病的影响。方法:用间接ELISA法测定18例阿尔茨海默病(AD)病人和20例正常对照者血浆中抗单纯疱疹病毒1型(HSV1)特异性IgG,IgM类抗体及抗单纯疱疹病毒2型(HSV2)特异性IgG,IgM类抗体;通过直接测序法检测ApoE基因的基因型并分析其单核苷酸多态性。结果:AD病人血浆中抗HSV1特异性IgG类抗体阳性率较正常对照明显升高(P<0.05);两组血浆中均未检测到抗HSV1,HSV2特异性IgM类抗体。含ε4等位基因的基因型频率在AD和对照组之间有明显差异(P<0.05);AD的发病与HSV1感染和携带ApoEε4有明显相关性(P<0.01,比值比(OR)1.809,4.976。结论:HSV1感染和携带ApoEε4对AD发病起到一定作用,两种因素同时存在可能是AD发病的危险因子。Objective： To explore the interaction between herpes simplex virus （HSV）infection and the single nucleotide polymorphism of apolipoprotein E （APOE） gene and the development of Alzheimer disease（AD）. Methods： Serum was collected and DNA was extracted from peripheral blood of 18 AD patients and 20 normal elderly people. PCR amplification was used to detect the host APOE gene, and then screened for genetic polymorphisms or mutations in APOE gene by DNA direct sequencing. The serum was used to determine anti-HSV1 IgG,IgM antibodies and anti-HSV2 IgG,IgM antibodies by ELISA. Results： The HSV1 IgG-positivity of AD patients was far higher than that of normals（P〈0.05） ;The ε4/4 and ε3/4 genotypes in AD group were significantly higher than those in the controls （P〈0.05）; The association of APOE ε4 with AD was significant in the HSV1 IgG-positive patients （P〈0.01, OR （1.809 4.976）. Condusions： These findings suggest that the combination of anti-HSV1-positive in brain and carriage of an APOE-epsilon 4 allele is a strong risk factor for AD development.

关 键 词：阿尔茨海默病 单纯疱疹病毒1型 人 免疫球蛋白G 免疫球蛋白M 抗体 病毒 载脂蛋白E类 

分 类 号：R392-33[医药卫生—免疫学] R742.3[医药卫生—基础医学]