用单细胞凝胶电泳法研究慢性染砷对子代大鼠海马DNA的影响  

An Observation with Single Cell Gel Electrophoresis on DNA Iinjury of Hippocampus Neurons in Filial Generation Rats Induced by Chronic Arsenic Poison

作　　者：张敏[1] 胡晓霞[1] 陈腾祥[1] 陈粲[1] 杨勤[2] 李淑芳[3] 易必达[1] 

机构地区：[1]贵阳医学院生理学教研室,贵州贵阳550004 [2]贵阳医学院病理生理学教研室,贵州贵阳550004 [3]贵阳医学院药理学教研室,贵州贵阳550004

出　　处：《贵阳医学院学报》2005年第5期423-425,共3页Journal of Guiyang Medical College

摘　　要：目的:以单细胞凝胶电泳法(SCGE)研究慢性染砷对子代大鼠海马神经DNA的影响。方法:从妊娠期前2周开始,用不同剂量的含砷水和自来水分别饲种鼠及其子代大鼠。子鼠染砷7个月后(从娩出后计算)取子鼠的海马组织制备单细胞悬液,进行SCGE实验。结果:长期慢性染砷对子代大鼠海马组织DNA具有显著的损伤作用,长期高剂量染砷损伤主要以中等程度损伤为主,低剂量染砷损伤以低度损伤为主,统计分析结果提示,高染砷组相对于对照组差异显著(P<0.01)。结论:通过SCGE实验,观察到慢性染砷可引起海马组织的细胞DNA损伤或损伤易感性增加。Objective： To investigate the DNA injury of neurons in hippocampus of filial generation rats induced by chronic poison of arsenic. Methods-Water with arsenic of different doses was given to matronymic rats in 2 weeks before pregnancy. Rats drank water without arsenic were the control group. When the filial generation rats were seven month old （from the birth）, the hippocampus tissues of them were collected and prepared to single-cell suspension, and then, DNA was assayed with single cell gel electrophoresis. Results： The DNA in hippocampus was significantly injured by chronic arsenic poison. The injury degree was low in the low arsenic dose group, and was moderate degree in high arsenic dose group. The injury was significantly more serious in high arsenic dose group than those in control group （P 〈 0.01）. Conclusion： Chronic poison of arsenic might cause hippocampus DNA in- jury or might increase injury susceptibility in filial generation rats.

关 键 词：砷中毒 海马 DNA 彗星试验 大鼠 

分 类 号：R995[医药卫生—毒理学]