甲基睾酮-KMnO_4-Ru(bipy)_3^(2+)-Ce(Ⅳ)化学发光检测甲基睾酮的研究  

Chemiluminescence Determination of Methyltestosterone with Methyltestosterone-KMnO_4-Ru(bipy)_3^(2+)-Ce(Ⅳ) System

作　　者：曾丹云[1] 吉邢虎[1] 何治柯[1] 

机构地区：[1]武汉大学化学与分子科学学院,武汉430072

出　　处：《分析科学学报》2005年第5期504-506,共3页Journal of Analytical Science

基　　金：国家自然科学基金(No.202750287);国家"863"计划项目(No.2002AA2Z2004)

摘　　要：在KMnO4存在下甲基睾酮可增强三(2,2′-联吡啶)钌(Ⅱ)[Ru(bipy)32+]-Ce(Ⅳ)化学发光体系的发光强度,基于此建立了一种化学发光检测甲基睾酮的新方法。在优化的实验条件下,该方法的线性范围和检出限分别为1.0×10-7~4.0×10-6mol.L-1和5.0×10-8mol.L-1。该法用于药片中甲基睾酮含量的测定,结果满意。文中还提出了可能的化学发光机理。It was observed that methyhestosterone could greatly enhance the chemiluminescence（CL） intensity of KMnO4-tris-（2,2＇-bipyridyl） ruthenium（ Ⅱ ） （Ru（bipy）3^2＋ ）-cerium（ Ⅳ ） （Ce（ Ⅳ ）） system, based on this fact,a novel CL method was developed for the determination of anabolic steroid. Under the optimum experimental conditions, the linear range and detection limit of the × 10^-7 - 4. 0 × 10^-6 tool · L^-1 and 5. 0 × 10^-8 mol · L^-1 , respectively. The developed method has been successfully applied to the determination of methyltestosterone in the troche. The mechanism of CL reaction was also studied.

关 键 词：甲基睾酮 化学发光 三(2 2'-联吡啶)钌(Ⅱ) 

分 类 号：O657.39[理学—分析化学] R917[理学—化学]