阿达玛变换显微荧光成像在单细胞定量分析中的应用研究  

Application of Hadamard Transform Microscopic Fluorescence Imaging to Single Cell Quantitative Analysis

作　　者：罗美娜[1] 李涛[1] 潘丽[1] 唐宏武[1] 

机构地区：[1]武汉大学化学与分子科学学院测试中心,武汉430072

出　　处：《分析科学学报》2005年第5期507-509,共3页Journal of Analytical Science

基　　金：国家自然科学基金(No.20475041)

摘　　要：本文探讨了用阿达玛变换(HT)显微荧光成像系统获得基于256灰度级图像的单细胞荧光强度和解码定标值之间的关系,将归一化的HT图像用于定量分析,建立了适合不同样品的定量分析方法。在此条件下,定量分析数据有很好的准确度和精密度;将系统用于单个乳腺肿瘤细胞的DNA定量分析,对乳腺肿瘤的良恶性及癌变程度进行判断,分析结果与病理学诊断结论一致。In this study,the relationship between the measured intensity of the single cells and the given decoding standard for the 256-gray-level image （HT image） obtained by Hadamard transform （HT） fluorescence imaging system was investigated, the normalized HT images were used for cellular quantitative analysis of different samples. On this condition,the quantitative data has good accuracy and precision,the system was applied to quantify the DNA ploidy in single breast tumor cells and to evaluate the proliferation degree of the breast tumor,and the result is in accordant with pathological diagnosis.

关 键 词：阿达玛变换 显微镜成像 乳腺肿瘤 单细胞定量分析 DNA倍性 

分 类 号：O657.32[理学—分析化学] Q-336[理学—化学]