Apelin激活大鼠主动脉阳离子氨基酸转运体的表达  

Apelin activates gene expression cationic amino acid transporter in isolated aorta of rat

作　　者：贾月霞[1] 杨靖辉[2] 潘春水[2] 张靓[2] 耿彬[2] 赵晶[2] 唐朝枢[2] 齐永芬[2] 

机构地区：[1]宁夏医学院,宁夏银川750004 [2]北京大学医学部,北京100083

出　　处：《宁夏医学杂志》2005年第10期655-657,共3页Ningxia Medical Journal

基　　金：国家重大基础发展规划项目(973)(No.G2000056905);国家自然科学基金(No:30470693);宁夏自然科学基金资助项目(No:NZ0542)

摘　　要：目的研究Apelin对大鼠离体主动脉阳离子氨基酸转运体(CAT)和诱导型一氧化氮合酶(iNOS)基因表达的影响。方法体外血管薄片孵育,测定孵育液中亚硝酸盐(NO2-)含量、RT-PCR方法测定阳离子氨基酸转运体CAT-1、CAT-2B和iNOS mRNA表达水平。结果大鼠主动脉孵育3h,Apelin(10-9-10-7mol/L)呈浓度依赖地刺激血管NO2-生成增加(33%、46%和69%,均P<0.01)。Apelin刺激血管L-Arg转运体CAT-1和CAT-2B mR-NA基因的表达上调,分别较对照组增加1.11倍和1.28倍(均P<0.01),而iNOS mRNA的表达并无明显改变(P>0.05)。结论Apelin激活血管组织阳离子氨基酸转运体CAT-1和CAT-2B基因表达,其变化可能参与了NO的释放从而引起血管的扩张。Objective Inthispresemsmdy, we assess the gene expression of cationlc amino acid transporter- l（CAT-1）, CAT- 2B and inducible nitric oxide syntheses （ilxg3S） in Apelin - stimulated rat thoracic aorta. Methods The aortic slices were incubated in vitro. Twelve rats were randomized to either a normal group or apelin group. The aortic NO production was detemained the medium nitrite content. Semi- quantitative RT- PCR was used to determine the rnRNA concentratioms of iNOS andCAT - 1 and CAT- 2B in aorta. Results After incubation of aorta for 3 hour, apelin（10^-9 - 10^-7 mol/l.） increased nitrite production （33 %, 46 % and 69 %, all P 〈 0.01 ） in dose dependent manner. Apelin - induced CAT- 1 and CAT- 2B rnRNA up - regulation and iNOS mRNA unchanged in aorta. Conclusion We provide the first evidence to illustrate that Apelin induces the trarkscription of high - affinity CAT- 1 and CAT- 2B in aorta.Transcription of CAT- 1 and CAT- 2B correlates well with aortic NO biosynthesis.

关 键 词：APELIN L-精氨酸 一氧化氮合酶 阳离子氨基酸转运体 

分 类 号：R96[医药卫生—药理学]