坎地沙坦对家兔快速心房刺激所致缝隙连接蛋白40重构的影响  

Effect of Candesartan on Connexin 40 Remodelling Reduced by Rapid Pacing in Rabbit Atrium.

作　　者：张家明[1] 李大强[1] 于世龙[1] 冯义柏[1] 胡还忠[2] 

机构地区：[1]华中科技大学协和医院心内科华中科技大学同济医学院心血管病研究所,湖北武汉430022 [2]华中科技大学同济医学院生理系

出　　处：《中国心脏起搏与心电生理杂志》2005年第5期385-387,共3页Chinese Journal of Cardiac Pacing and Electrophysiology

摘　　要：观察血管紧张素Ⅱ受体1拮抗剂坎地沙坦(Candesartan)对快速心房起搏刺激时家兔心房肌连接蛋白40(Cx40)重构和组织钙(Ca2+)含量的影响,探讨其防治心房颤动的可能机制。32只家兔随机分为3组:对照组(n=8)、快速刺激组(Ⅰ组,n=12)、Candesartan+快速刺激组(Ⅱ组,n=12)。经颈内静脉将电极置入右房,对照组不给予心房刺激,后两组以600次/分行快速心房刺激,并且Ⅱ组于快速刺激前30min开始按0.5mg·kg-1·min-1分持续静脉给予Candesartan8h,另外两组则给予等量的生理盐水。用免疫荧光标记激光共聚焦显微镜检测Cx40的含量和分布,用生化方法检测右心耳组织Ca2+含量。结果:与对照组相比,Ⅰ组和Ⅱ组心房组织Cx40含量降低(0.83±0.14μm2/μm3,2.05±0.36μm2/μm3vs2.35±0.26μm2/μm3,P<0.01或0.05)、分布不均一,Ⅰ组Ca2+含量升高(5.5±1.6μmol/gvs2.9±0.8μmol/g,P<0.01);与Ⅰ组相比,Ⅱ组心房Cx40含量增加(P<0.01)、分布不均一的程度减轻,Ca2+含量降低(3.2±1.1μmol/gvs5.5±1.6μmol/g,P<0.01)。结论:坎地沙坦能有效减轻心房快速刺激所致Cx40重构,其机制之一可能与降低心房Ca2+含量的异常升高有关。The pourse of this study was to observe the effects of Candesartan on connexin 40 remodelling induced by rapid pacing in rabbit atrium. 32 rabbits were randomly divided into 3 groups： control group（n = 8, rapid pacing group （Group Ⅰ , n = 12） , rapid pacing combining with Candesartan group （Group Ⅱ , n = 12）. Rapid pacing was performed in the last two groups at the frequency of 600 beats per minute for 8 hours. The infusion of Candesartan was initiated 30 minutes before rapid pacing in Group Ⅱ and the same volume of saline was infused in the other two groups. Atrial tissue was obtained from right atrial appendages to assess Ca^2＋ content and Cx40 level. Distribution of Cx40 was detected by confocal fluorescent electron microscope. Results： In Group Ⅰ , contents of Ca^2＋ and Cx40 were significantly higher and lower than those in control group respectively （ P 〈 0.01 ）, and the distribution pattern of Cx40 was inhomogeneous. In Group Ⅱ , the changes Cx40 were similar to those in Group I as compared with control group （P 〈 0.05）, but the levels of Ca^2＋ decreased and Cx40 increased significantly than those in Group Ⅰ respectively （P 〈 0.01 ）, and the alleviation of inhomogeneity in the Cx40 distribution was observed as well. Conclusion： Candesartan can effectively alleviate Cx40 remodelling induced by rapid pacing in rabbit atrium. One mechanism of this effect is associated with attenuation of calcium overload in atrium. [ Chinese Journal of Cardiac Pacing and Electrophysiology,2005,19 （5） ：385 - 387 ]

关 键 词：心血管病学 心房颤动 缝隙连接蛋白重构 坎地沙坦 钙超载 

分 类 号：R971.94[医药卫生—药品] R541.75[医药卫生—药学]