在内皮素-1诱导增殖的血管平滑肌细胞中骨桥蛋白的表达  

Expression of Osteopontin in Proliferated Human Umbilical Artery Smooth Muscle Cell Induced by Endothelin-1

作　　者：綦惠[1] 商战平[2] 王家富[2] 

机构地区：[1]北京大学医学部生理系 [2]山东省泰山医学院

出　　处：《中西医结合心脑血管病杂志》2005年第10期871-874,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：山东省自然科学基金项目(No.Z2002C40)

摘　　要：目的观察在内皮素-1(ET-1)诱导增殖的人脐动脉平滑肌细胞(HUASMC)中骨桥蛋白(OPN)表达的变化。方法组织贴块法培养HUASMC后;随机分成5组,组1、组2、组3分别给予ET-1100nmol/L、10nmol/L、1nmol/L刺激48h,组4为不加ET-1的对照组,组5给予10μmol/LBQ123(ET-1A型受体的特异性拮抗剂)预处理后,再用10nmol/LET-1刺激48h,MTT比色法检测细胞在490nm的吸光度值(OD490),组间进行比较;免疫细胞化学方法检测OPN在不同组之间的表达,计算并比较表达的阳性率;分析OPN表达与HUASMC增殖的相关关系。结果ET-1剂量依赖性刺激HUASMC增殖,组间OD490比较有统计学意义(P<0.01),BQ123可以抑制ET-1的作用;随ET-1浓度的升高,OPN表达阳性率升高(P<0.01),给予BQ123预处理后,OPN表达的阳性率亦显著低于单独ET-1刺激组(P<0.01)和对照组(P<0.05);OPN表达阳性率与OD490值之间存在直线正相关(r=0.962,P<0.01)。结论ET-1剂量依赖性刺激血管平滑肌细胞(VSMC)增殖,在ET-1诱导增殖的VSMC中OPN表达增加,OPN表达和HUASMC增殖之间存在直线正相关关系,检测VSMC中OPN的表达,对于VSMC增殖情况有一定预测价值。Objective To study the variation of the expression of Osteopontin （OPN） in the proliferated human umbilical artery smooth muscle cell （HUASMC） induced by ET - 1. Methods ①HUASMCs were cultured by explant method ; ②Cultured HUASMCs were divided into 5 groups randomly：Group 1, group 2, group 3 （treated with 100 nmol/L, 10nmol/L, and 1nmol/L ET- 1 respectively）, group 4 without treatment by ET- 1, and group 5 （pretreated with 10μmol/L BQ123 and then cultured in 10nmol/L ET- 1 ）. All of the cells were cultured for 48 hours. The level of 013490 was evaluated in different groups by MTT assay;③The expression of OPN was measured by immunocytology, and the positive rate was compared among groups; ④The relationship between the proliferation of HUASMC and the expression of OPN was analyzed. Results The higher the concentration of ET - 1, the higher of 013490, and the differences were significant （P〈 0.01）. ET - 1 increased the expression of OPN dose - dependently （ P 〈 0.01 ）. The expression of OPN was correlated with the proliferation of VSMC（ r = 0. 962, P 〈 0.01 ）. Conclusion ET - 1 could promote the proliferation of VSMC dose - dependently. OPN expression increased significantly after treated with ET - 1. OPN expression was correlated with VSMC proliferation positively. The examination of OPN might indicate the early proliferation of VSMC.

关 键 词：内皮素-1 血管平滑肌细胞 骨桥蛋白 

分 类 号：R543.3[医药卫生—心血管疾病]