北京地区呼吸道合胞病毒分离株F蛋白基因序列分析  

Sequence Analysis of F Protein Gene of Respiratory Syncytial Virus in Beijing Area

作　　者：芦起[1] 申昆玲[1] 张燕[2] 刘亚谊[1] 赵宇红[1] 刘春艳[1] 姬奕新[2] 谢正德[1] 许文波[2] 

机构地区：[1]首都医科大学附属北京儿童医院病毒室 [2]中国疾病预防控制中心病毒疾病预防控制所国家麻疹室

出　　处：《首都医科大学学报》2005年第5期565-567,共3页Journal of Capital Medical University

摘　　要：目的分析中国北京地区4株呼吸道合胞病毒(RSV)F蛋白的基因序列并与RSV原型株A2进行比较,得出中国北京地区RSV F蛋白基因是否存在变异及变异特征.方法用反转录聚合酶链反应(RT-PCR)分2段扩增F蛋白的基因核苷酸序列,将得到的P1、P2 2段切胶纯化标记后测序,应用Bioedit软件对得到的序列做碱基配对和比较分析.结果4株RSV F蛋白核苷酸序列的同源性为94.92%～95.62%.信号肽区的同源性为99.36%～99.73%.F1、F2亚单位同源性分别为98.93%～99.31%和96.69%～97.33%.氨基酸同源性分别为:F蛋白的蛋白编码区97.39%～97.74%,信号肽区99.13%～99.65%,F1、F2亚单位98 78%～99.48%、98.61%～99.30%.结论中国RSV北京株与原型株A2之间的F蛋白基因变异小,具有很高的同源性.氨基酸在抗原位点Ⅱ保守,此段氨基酸可作为RSV疫苗的候选位点.Objective To study the sequence of F protein gene of 4 respiratory syncytial virus strains in Beijing area of China. Methods Pt and P2 segments of respiratory syncytial viruses were amplified by RT-PCR, then the PCR products were purified for sequencing. Analysis was carried out by Bioedit program. Results The nucleotide sequences of the four RSV strains F mRNA had 94.92 % -95.62 % overall nucleotide identity with those of RSV isolate A2, the nucleotide sequences of signal peptide of four RSV strains F mRNA had 99.36 % - 99.73 % identity, the F1 chain had 98.93 % - 99.31%, F2 chain had 96.69 % - 97.33 % identity respectively. The deduced aminoacid sequences of the four strains and A2 F proteins had 97.39 % - 97.74% overall aminoacid identity, the signal peptide had 99.13 % -99.65 %, the F1 chain had 98.78 % -99.48 %, F2 chain had 98.61% - 99.30% identity respectively. Conclusion The differences between the A2 and Beijing strains in the F gene are small, so the nucleotide and aminoacid have high identity. The antigenic area Ⅱ could be the potential site of RSV vaccine.

关 键 词：呼吸道合胞病毒 F蛋白 核苷酸序列 

分 类 号：R725.6[医药卫生—儿科]