旱柳Q106组织培养及快繁体系的建立  

Tissue culture and rapid propagation of Salix matsudana Q106 in vitro

作　　者：余如刚[1] 杜雪玲[1] 夏阳[2] 梁慧敏[2] 李阳春[3] 

机构地区：[1]安徽淮北煤炭师范学院,安徽淮北235000 [2]山东省林业科学研究院,山东济南250014 [3]甘肃农业大学草业学院,甘肃兰州730070

出　　处：《草原与草坪》2005年第5期57-59,共3页Grassland and Turf

基　　金：山东省科技发展计划(011100104);国家"863"计划(2001AA244082)资助

摘　　要：以旱柳(Salixmatsudana)Q106春季萌生嫩枝为外植体,建立了“以芽繁芽”的组织培养快繁体系。丛生芽培养基为MS+BA0.4mg/L+NAA0.2mg/L。继代培养基为MS+BA0.4mg/L+NAA0.2mg/L和BA0.4mg/L+NAA0.4mg/L;MS+BA0.4mg/L+NAA0.2mg/L和BA0.4mg/L+IBA0.2mg/L交替使用。最佳生根培养基为MS+GA30.2mg/L+NAA0.5mg/L。Rapid propagation system of Salix matsudana Q106 was established by using one axillary bud stem as explants which derived from internodal sections of 1-year-old Salix matsudanaQl06. The results obtained as follows： the axillary bud can obtain better multiplication on MS ＋0.4 mg/L BA ＋0.2 mg/L NAA culture,while developed plants were sub-cultured on MS＋0.4 mg/L BA＋0.2 mg/L NAA and 0.4 mg/L BA＋ 0.4 mg/L NAA or MS＋0.4 mg/L BA＋0.2 mg/L NAA alternate with 0.4 mg/L BA＋0.2 mg/L IBA culture. The optimum culture for rooting is MS＋0.2 mg/L GAa ＋0.5 mg/L NAA.

关 键 词：旱柳Q106 组织培养 激素 

分 类 号：Q943.1[生物学—植物学]