络合分光光度法测定卡铂抗肿瘤膜剂中卡铂含量  

Determination of carboplation in films by chelating reaction-spectrophotometry

作　　者：孔维涵[1] 赵博[2] 陈启蒙[3] 李璐[3] 韩志霞[3] 

机构地区：[1]天津市天津医院,天津300211 [2]天津医科大学总医院,天津300052 [3]天津医科大学,天津300070

出　　处：《天津药学》2005年第5期17-18,共2页Tianjin Pharmacy

摘　　要：目的:建立络合分光光度法测定膜剂中卡铂含量.方法:用蒸馏水提取膜剂中卡铂,与等体积1 mol/L SnCl2的1 mol/L盐酸溶液反应后,用络合分光光度法测定反应液吸收度,检测波长为395 nm.结果:反应液中卡铂浓度于7.67～46.00 μg/ml之间与其吸收度有良好线性关系,r=0.999 3;平均回收率＝100.3%,RSD＝2.3%(n=6).结论:该方法简便、快速、准确,可用于卡铂膜剂质量控制中的卡铂含量测定.Object ： To establish a chelating reaction-spectrophotometry method for the determination of carboplation in films. Methods： Carboplation in films was extracted by distilled water, and reacted with the same volumes of 1 mol/L SnCl2 and 1 mol/L HCl. Chelating reaction-spectrophotometry was used for the determination. The detection wavelength was set at 395 nm. Results： The method was linear within the range of 7.67～46.00μg/ml（ r =0.9993）. The average recoveries were 100.3% with RSD of 2.3% （n=6）. Conclusion： The method was simple, rapid and accurate for the determination of carboplation in films. It was reliable for the quality control of the films.

关 键 词：卡铂 膜剂 络合分光光度法 

分 类 号：R927.2[医药卫生—药学]