液质联用法对两种虫草中核苷类成分的研究  

Study on nucleotide in stroma of Cordyceps sp.by HPLC-MS

作　　者：陈畅[1] 罗珊珊[1] 史艳秋[1] 孙迎节[1] 张长铠[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,山东济南250100

出　　处：《中国生化药物杂志》2005年第5期260-263,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：山东省科技攻关计划项目课题(No.012100104)

摘　　要：目的优化色谱条件,在最佳条件下对蒙山九州虫草和冬虫夏草子座中核苷类化合物进行定性和定量研究,并比较两种虫草的不同。方法用高效液相色谱(HPLC)法对供试品中核苷类成分进行初步定性和定量的研究,然后用质谱(MS)法进行成分验证。结果HPLC与MS法联用能准确地对各组分进行定性和定量分析;洗脱液的最佳配比为:先以99%水+1%乙腈梯度洗脱5 min后过渡为96%水+4%乙腈至洗脱完全。结论首次将此方法用于虫草中核苷类成分的研究,发现蒙山九州虫草含有比冬虫夏草种类更多的核苷类成分,有望替代价格昂贵的冬虫夏草应用于临床。Purpose To optimize the chromatogram condition, to study nucleotides in stroma of Cordyeeps sinensis and Cordyceps kyushuensis, and to compare the nucleotide contents of the two Cordyceps sp. Methods The qualitative and quantitative nucleotides in the stroma of Cordyceps sp. were analysed by HPLC-MS. Results The contents of the nucleotides in the samples were exactly identified and quantitated. The samples were ananlysed according to the following gradient program：0-5 min： from 99 % H2O＋1% ACE to 96 % H2O＋4% ACE,5-30 min：96% H2O＋4% ACE. Conclustion HPLC-MS is used for the first time to study nueleotide in stroma of cordyceps sp. Cordyceps kyushuensis has good prospect, and can also be expected to replace Cordyceps sinensis clinically.

关 键 词：核苷类 高效液相色谱-质谱联用 虫草 

分 类 号：R282.71[医药卫生—中药学] R917[医药卫生—中医学]