人体血中美百乐镇（PRAVASTATIN）浓度的气相色谱／质谱测定法  

The Quantitative Method of Pravastatin in Plasma by Gas Chromatograoahy/Mass Spectrometry

作　　者：蔡葵花[1] 谭炳炎[1] 冯智英[1] 李章旺[1] 黄民[1] 赵香兰[1] 

机构地区：[1]中山医科大学中心实验室,中山医科大学临床药理教研室

出　　处：《色谱》1996年第2期121-123,共3页Chinese Journal of Chromatography

摘　　要：报道了采用乙酸乙酯萃取纯化样品，经重氮甲烷酯化、ＢＳＴＦＡ硅烷化后，用气相色谱－质谱法的选择离子检测（ＳＩＭ）定量模型，对ｐｒａｖａｓｔａｔｉｎ的血药浓度进行测定的方法。方法的最低检出限为０．０１５ｎｇ，在１．０～６０ｎｇ／ｍＬ的浓度范围内，标准曲线呈良好的线性关系，ｒ＞０．９９，ＣＶ＜７．７％。Pravastatin sodium is a cholesterol-lowering agent and potent inhibitor of 3hydroxy-3-methylglutaryl coenzyme A (HMGCoA) reductase. The plasma concentration is very low. This paper reports a highly sensitive and quantitative method specific for determination of pravastatin in human plasma based on the selective ion monitoring (SIM) mode in gas chromatography/electron impact ionization mass spectrometry. The plasma samples were acidified to pH 4. 0 and then extracted by ethyl acetate. The plasma concentrations of pravastatin in healthy volunteers were detected by selecting m/z 462 as quantitation ion after the sample was methyl esterification with diazomethane and silanization with BSTFA. The minimun detectable limit was 0. 015ng. The standard curve was linear between 1.0 to 60 ng/mL, with a correlation coefficient more than 0. 99. The coefficiens of variation was less than 7.7%. The recovery can reach 90%. This method has been successfully used for the study of pharmacokinetics of pravastatin.

关 键 词：气相色谱-质谱法 美百乐镇 血药浓度 药代动力学 

分 类 号：R446.11[医药卫生—诊断学] O657.71[医药卫生—临床医学]