同型半胱氨酸对血管内皮细胞的氧化应激损伤及对IL-8分泌的影响  

Homocysteine induces oxidative stress injury and IL-8 secretion in vascular endothelial cells in vitro

作　　者：张铭[1] 陈怀生[1] 杨大春[1] 曾智[1] 余敏[1] 

机构地区：[1]四川大学华西医院心内科,四川成都610041

出　　处：《心脏杂志》2005年第5期450-452,共3页Chinese Heart Journal

摘　　要：;目的:通过观察病理浓度下的同型半胱氨酸HCY对培养的内皮细胞(EC)损伤及其氧化应激的影响。同时检测IL-8的表达,探讨HCY在动脉粥样硬化(AS)中的作用。方法:体外培养人脐静脉血管EC ECV-304。测定氧化应激损伤指标,分正常对照组和HCY组(浓度分别为50、100μmol/L、1.0、2.5、5.0 mmol/L组)。测细胞因子IL-8的分泌表达,分正常对照组和HCY组(浓度分别为50、200μmol/L组),孵育0.5、8、24 h后,测IL-8表达。结果:高浓度HCY对EC损伤明显,IDH值升高,活性氧和MDA水平增高,而SOD,CAT活性下降,NO的生成减少。IL-8的表达量增加,且分泌高峰提前出现。结论:病理水平HCY可以增强氧化应激损伤反应,刺激EC分泌IL-8。AIM： To observe the effect of pathological level of homocysteine （HCY） on cells and oxidative stress and the secretion of IL-8 in endothelium. METHODS： 1. ECV304 human umbilical endothelial cells were incubated in vitro and treated with HCY at diffent concentrations. The levels of CAT, NO, SOD,MDA were determined. The expression of IL-8 was assessed by enzyme-linked immuno-sorbent assay. RESULTS：① High HCY caused cell injuries, increased LDH,activate oxygen and MDA levels. while the levels of SOD, CAT and NO were reduced.② High HCY induced the expression of IL-8. CONCLUSION：Pathologicial level of HCY could impair endothelial cells, induce oxidative stress injury and IL-8 secretion of endothelial cells.

关 键 词：同型半胱氨酸 氧化应激损伤 动脉粥样硬化 白细胞介素-8 

分 类 号：R543.12[医药卫生—心血管疾病]