ELISA检测心磷脂抗体在胎儿发育方面的应用  

Application of Serum Anticardiolipin Antibody (ACA) Determination with ELISA for Detection of Intrau-Uterine Growth Retardation (IUGR)

作　　者：陈文[1] 钟建辉[1] 沈均南[1] 

机构地区：[1]广东省肇庆市第一人民医院,526060

出　　处：《放射免疫学杂志》2005年第5期395-396,共2页Journal of Radioimmanology

摘　　要：目的:探讨胎儿生长迟缓(IUGR)与抗心磷脂抗体(ACA)的相关性。方法:用酶免疫吸附法ELISA检测5330例正常孕妇血清的ACA,同时对IUGR合并ACA阳性产妇产后胎盘进行免疫荧光检查,了解其结构改变。结果:正常孕妇ACA阳性发生率为2.70%,其中IUGR发生率15.28%,ACA阴性的孕妇IUGR发生率为1.77%,两者比较,差异有极显著性(P<0.01)。新生儿IUGRIgG阳性5例,胎盘免疫荧光检查发现胎盘组织均出现免疫球蛋白荧光抗体和补体阳性着色。结论:ACA阳性是IUGR发生的病因之一,ACA检测给IUGR的病因诊治提供了新的研究途径。Objective To explore the interrelationship between presence of serum ACA and development of IUGR. Methods Serum ACA contents were examined with ELISA in 5330 apparently normal pregnant women. The placentae in 16 pregnancies with IUGR and positive ACA were examined with immunofluroscence microscopy. Results Among the 5330 pregnant women there were 144 with positive ACA （2.70%） , in whom there were 22 IUGR cases （22/144, 15.28% ）. The rate of IUGR in the remaining ACA negative women was only 1.77 % （92/5186 ）, the difference being very significant （ P 〈 0.01 ）. ACA IgG was positive in 5 of the IUGR neonates, All the placental tissues examined with immunofluroscence microscopy showed positive immunoglobulin fluroscence antibody and complement discolouration. Conclusion Positive ACA is one of the causative factors of development of IUGR and determination of ACA in pregnant women was helpful for early detection of IUGR.

关 键 词：胎儿生长迟缓 抗心磷脂抗体 ELISA检测 抗心磷脂抗体 胎儿发育 ACA检测 免疫荧光检查 IUGR 酶免疫吸附法 胎儿生长迟缓 胎盘组织 

分 类 号：R512.62[医药卫生—内科学] R714.21[医药卫生—临床医学]