草鱼生长激素单克隆抗体杂交瘤细胞系的建立及鉴定  

THE DEVELOPMENT AND IDENTIFICATION OF HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODIES AGAINST GRASS-CARP GROWTH HORMONE

作　　者：杨峰[1] 樊震[1] 王远程[1] 陈松林[2] 贺路[2] 

机构地区：[1]中国农业科学院生物技术研究中心,北京100081 [2]中国水产科学研究院长江水产研究所,沙市434000

出　　处：《微生物学报》1996年第1期25-30,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金资助项目

摘　　要：用经草鱼生长激素(gcGH)免疫的BALB/c小鼠脾细胞与小鼠骨髓瘤细胞(Sp2/O-Ag14)融合,经3次克隆化培养和ELISA筛选,得到7株阳性杂交瘤细胞株,其中2株为强阳性。交叉试验表明,这些杂交瘤细胞株所分泌的单克隆抗体(McAb)同大鳞大马哈鱼生长激素(sGH)、大鳞大马哈鱼促性腺激素(sGTH)及牛生长激素(bGH)均不起反应。小鼠腹水McAbs滴度高达1:1280000。受体-gcGH-McAb夹心试验表明,McAb确与有活性的gcGH有特异反应。用间接ELISA方法可检测到浓度为0.125#g/ml的gcGH。Monoclonal antibodies of hybridomas derived from fusing the mouse spleencytes immunized by gcGH with BALB / c mouse myeloma cells (Sp2 / 0-Agl4).Seven hybridomas cell lines were produced by indirect-ELISA screening and cloning for three times with limited dilution.In crossreaction test, these monoclonal antibodies didn't react with sGH, sGTH and bGH. Antibody liter of ascitic fluids was about 1 : 1280000 in indirect-ELISA. In receptor-gcGH-McAb sandwich-ELISA test, monoclonal antibodies reacted with biological-active gcGH. GcGH can be detected at the concentration of 0.125 /μg/ ml in indirect-ELISA.

关 键 词：草鱼 生长激素杂交瘤 单克隆抗体 农业生物工程 

分 类 号：S965.112[农业科学—水产养殖] S188[农业科学—水产科学]