流行性乙型脑炎14-2株病毒在乳狗肾细胞上的适应性  

Adaptability of Strain_(14-2) of Japanese Encehalitis Virus in Suckling Dog Kidney Cells

作　　者：王叔桥[1] 王寿贵[1] 李玉华[1] 

机构地区：[1]卫生部成都生物制品研究所,成都610063

出　　处：《中国生物制品学杂志》1996年第1期18-20,共3页Chinese Journal of Biologicals

摘　　要：流行性乙型脑炎14－2株弱病毒在乳狗肾细胞上连续传11代，1代的病毒滴度即达6．21LogPFU／ml，1代后各代病毒滴度无显著升高（6．18～6．40LogPFU／ml）。传代后的病毒对3周龄小鼠脑内无毒力，皮下无致病力，经乳鼠脑内传代后的脑内毒力为1．5～3．0LogLD5／0．03ml，与14－2原株的持世无显著差异。用乳狗肾细胞为基质制备的冻干活疫苗，经全面检定完全符合现行《乙型脑炎活疫苗规程》标准。The strain14-2 of Japanese encephalitis virus has been serially undergone 11 pasages in suckling dog kidney cells. The virus titer of the strain reached 6. 21 Log PFU/ml after the first passage. However, when it was further passaged, no significant increase of titer was found. The passaged strain14-2, showed no intracerebral toxicity or subcutaneous pathogenicity to 3-week-old mice. On the contrary, the intracerebral toxicity of those passaged in sulkling murine brain was 1. 5~3.0 LogLD50/0.03ml and showed no significant difference compared with primary strain. Complete characterization and control tests proved that the quality of the freezeudried live JE vaccine using suckling dog kidney cells as substrate reached current live JE vaccine requirements.

关 键 词：乙型脑炎 14-2株病毒 乳狗肾细胞 适应性 疫苗 

分 类 号：R512.320.3[医药卫生—内科学] R392－33[医药卫生—临床医学]