药物对病毒感染培养心肌细胞Ca^（2＋）内流及CVB_3－RNA复制的影响  

Effect of Drugs on Ca￣(2+) Influx and CVB3-RNA Replication in Cultured Rat Heart Cells Infected with CVB3

作　　者：郭棋[1] 彭天庆[1] 杨英珍[1] 顾全保 赵剑星 

机构地区：[1]上海医科大学中山医院,上海市心血管病研究所卫生部病毒性心脏病重点实验室,中国科学院上海细胞生物学研究所

出　　处：《中国病毒学》1996年第1期40-44,共5页Virologica Sinica

基　　金：国家自然科学基金

摘　　要：应用放射性同位素￣（４５）Ｃａ￣（２＋）示踪技术及光敏生物素标记ｃＤＮＡ探针杂交方法，观察了维拉帕米、黄芪、地塞米松等药物对感染柯萨奇Ｂ＿３病毒（ＣＶＢ＿３）的大鼠培养心肌细胞Ｃａ￣（２＋）内流及细胞中ＣＶＢ＿３－ＲＮＡ复制的作用。结果发现：在病毒感染４８ｈ，上述三药均可显著减少感染细胞及正常对照的心肌Ｃａ￣（２＋）内流（Ｐ＜０．０１和Ｐ＜０．０５）；若在病毒感染后即加入上述药物，经４８ｈ培养后，黄芪组细胞中的ＣＶＢ＿３－ＲＮＡ含量显著少于病毒对照组（Ｐ＜０．００１），维拉帕米则显著增加（Ｐ＜０．０１）．而地塞米松对其无影响。提示黄芪与地塞米松具有一种与维拉帕米相似的减少病毒感染心肌Ｃａ￣（２＋）内流的作用，有可能减轻感染细胞的继发性Ｃａ￣（２＋）损伤；但三种药物对感染细胞中病毒核酸的复制有不同作用，可作为临床治疗病毒性心肌炎时的参考。he effect of Verapamil(Ver),Astragalus membranaceus(AM),Dexamethasone(Dex)on Ca￣(2+) influx across the myocardial plasma membrane and coxsackie virus B3(CVB3)-RNA replica-tion in cultured neonatal rat heart cells infected with CVB3 was investigated.It was found thatthe Ca￣(2+) influx could be decreased significantly (p< 0.01)by Ver,AM,Dex after infection for48h.In addtion,the aniounts of CVB3-RNA in cultured heart cells infected with CVB3、and treat-ed with AM for 48h simultaneously were significantly decreased as compared with the heart cellsinfected with CVB3 only(p< 0.001) and it was significantly higher in myocytes infected withCVB3 and treated with Ver than in myocytes only infected with CVB,(p< 0.01).These resultsdemonstrated that the above three drugs could exert the effect of decreasing the secondary dam-ages caused by Ca￣(2+)influx,but their effects on replication of CVB3-RNA in myocardium werequite different:AM inhibited replicantion of CVB3+RNA,Ver enhanced CVB3-RNA replication,while Dex did not affect the CVB3-RNA replication. The results suggest that AM is the bestchoice in treating patients with acute viral myocarditis.

关 键 词：病毒性心肌炎 柯萨奇B3病毒 维拉帕米 黄芪 

分 类 号：R542.210.5[医药卫生—心血管疾病] R965.1[医药卫生—内科学]