水稻非对称混合样品中非优势DNA模板SSR标记的PCR检测  

PCR Amplification of SSR Markers for Minor Templates in non-equal DNA Bulks in Rice

作　　者：陈宏伟[1] 罗利军[1] 李荧[1] 冯芳君[1] 吴金红[2] 陈亮[2] 徐小艳[2] 梅捍卫[2] 

机构地区：[1]华中农业大学植物科技学院,武汉430070 [2]上海市农业生物基因中心,上海201106

出　　处：《分子植物育种》2005年第5期731-736,共6页Molecular Plant Breeding

基　　金：农业部948项目(948-2001-101);上海市自然科学基金(02ZC14082);国家863计划(2003AA207010)共同资助。

摘　　要：利用DNA混合池筛选分子标记可以大大提高检测效率,来自不同亲本的模板DNA的浓度一旦相差悬殊,可能影响PCR扩增结果。本文对不同稀释浓度和混合比例的模板DNA进行SSR标记的PCR扩增。结果表明,单一模板不同稀释倍数的影响不明显,但不同模板DNA竞争造成非对称混合样中非优势模板扩增量的明显降低。对于扩增效果较好的SSR标记而言,模板浓度比为1:7时仍可检测到低浓度模板的扩增产物。Bulked DNA pool is used to increase the efficiency in screening of molecular markers. The PCR amplification is probably influenced if the contents of template DNAs from different parents were tremendously different in the bulks. PCR amplification of SSR markers was conducted by using different diluted or mixed template DNA samples in this study. It was shown that PCR products were not significantly decreased by dilution of one template DNA, but had a sharp decrease caused by the competition between different templates in non-equally mixed DNA bulks. The PCR products of minor content template DNA was detectable for well-amplified SSRs when the two template DNAs were mixed in a proportion of 1 ： 7.

关 键 词：DNA混合池 SSR标记 PCR扩增 水稻 DNA模板 PCR检测 混合池 非对称 优势 中非 

分 类 号：S511[农业科学—作物学]