炭凝集试验快速检测呼吸道合胞病毒的研究  

Study of Carbon Aggl utinaion Test for the Rapid Detection of Respiratory Syncytial Virus

作　　者：董继华[1] 江汉珍[1] 周生华[1] 谢红斌[1] 田慕贞[1] 

机构地区：[1]同济医科大学附属协和医院

出　　处：《中国病毒学》1996年第2期116-119,共4页Virologica Sinica

基　　金：湖北省科委重点资助课题

摘　　要：用炭凝集试验（ＣＡＴ）检测呼吸道合胞病毒（ＲＳＶ），结果表明该法是一种简便、快速、特异的诊断方法。用ＣＡＴ对１６株ＲＳＶ和８株其它病毒做试验，结果仅ＲＳＶ凝集，而其它病毒均阴性。用该法与细胞培养法检测８３份临床呼吸道感染幼儿鼻咽吸出物，结果ＣＡＴ法阳性率为６９．８８％（５３／８３），细胞培养法为３９．７５％（３３／８３），两者阳性检出率相差极显著。阻断试验证明ＣＡＴ是高度特异的。结果证明ＣＡＴ具有较高的敏感性与特异性，可用于临床ＲＳＶ标本的快速检测。his study reports a simple,rapid and special diagnosis method of carbon agglutination test(CAT)to detect Respiratory Syncytial Virus(RSV).Sixteen specimens of RSV were seen agglu-tination but 8 specimens of other virus were negative.We compared CAT with tissus culture byusing nasopnaryngeal secretions from children with suspected RSV iofection.Of 83 specimens,53(69.88%)were positive by CAT,33(39.75%)were positive by virus culture,There were verysignificant difference in the positive rates beween CAT and culture Blocking test showed thatCAT has high specificity.We believe that CAT has a high sensitivity and specificity and it can beused for the rapid detection of clinical specimens of RSV.

关 键 词：呼吸道合胞病毒 感染 炭凝集试验 诊断 

分 类 号：R560.4[医药卫生—呼吸系统]