用脉冲电场凝胶电泳研究γ射线诱导的DNA双链断裂  

Fragmentations in DNA Double-strand Breaks irradiated with Gamma Ray by PFGE

作　　者：臧黎慧[1] 魏志勇[1] 李明[1] 童建[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院,江苏苏州215007

出　　处：《中国血液流变学杂志》2005年第2期193-196,共4页Chinese Journal of Hemorheology

摘　　要：用不同剂量的60Coγ射线照射GM0639细胞,利用脉冲电场电泳仪测量DNA双链断裂后的断裂片段分布.结果表明在20～100Gy的照射剂量下, γ射线引起的DNA断裂片段释放的比例(FAR)随照射剂量的增加而增加,在剂量比较大时释放的比例(FAR)趋于饱和, DNA断裂片段的平均长度随照射剂量的增加而减小,60Coγ射线对GM0639细胞的断裂产额为(6.00±0.6)×10-9Dsb/Bp/Gy.GM0639 cells were irradiated by Gamma ray emitted from source ^60Co, and the yields and distributions of double-strand breaks （DSB） were measured by he method of pulsed-field gel electrophoresis （PFGE）. The results showed that the fractions of the activity released （FAR） of DNA increased with the increase of dose and ap- proached to a threshold （-85%） at larger doses, while the mean sizes of DNA fragments decreased when the dose increased. The yield of DSB of GM0639 induced by ^60Co Gamma rays was calculated as （6.00±0.6）×10^-9 Dsb/Bp/Gy.

关 键 词：DNA双链断裂 初始损伤 DSB产额 脉冲电场凝胶电泳 

分 类 号：R811.5[医药卫生—放射医学]