自体外周血造血干细胞移植后造血重建中CD226分子在NK细胞上表达的变化  

Expression of CD226 on NK Subsets During Reconstitution of Immune System by Autologous Peripheral Blood Hematopoietic Stem Cell Transplantation

作　　者：张赟[1] 程光[2] 尤向辉[3] 张红梅[3] 任军[3] 金伯泉[1] 

机构地区：[1]第四军医大学免疫学教研室,陕西西安710032 [2]第四军医大学西京医院神经外科,陕西西安710032 [3]第四军医大学西京医院肿瘤科,陕西西安710032

出　　处：《标记免疫分析与临床》2005年第3期152-154,134,共4页Labeled Immunoassays and Clinical Medicine

基　　金：国家自然科学基金资助项目(NO.39970688);重点课题资助项目(NO.30030130)

摘　　要：本文应用双重免疫荧光染色和流式细胞术分析,观察自体外周血造血干细胞移植患者造血重建中,CD226分子在CD56bright和CD56dimNK细胞亚群表达的变化。结果显示,在自体外周血造血干细胞移植造血重建中,于干细胞回输第12天,CD56+NK细胞占外周血淋巴细胞百分率为26.6%,其中CD56bright亚群,占CD56+NK细胞87.3%;CD56+CD226+细胞占CD56+NK细胞92.1%,CD56brightCD226+细胞占CD56+CD226+细胞89.9%。在自体外周血造血干细胞移植造血重建中,CD56bright亚群是NK细胞造血重建最早出现并占优势的一个亚群,CD226分子可能作为一种分化标记主要表达在CD56bright亚群上。The purpose of this paper was to observe the expression of CD226 on NK subsets during reconstitution of immune system by autologous peripheral blood hematopoietic stem cell transplantation. Double fluorescent staining and flow cytometry analysis were employed to detect the expression of CD226 on CD56^bright and CD56^dim NK subsets during reconstitution of immune system by autologous peripheral blood hematopoietic stem cell transplantation. The results showed that on day 12 after transplantation, the percentage of CD56^＋ NK cells in PBMC increased to 26.6%. Among CD56^＋ NK cells, the percentage of CD56^bright NK cells was 87.3% and that of CD56^＋ CD226^＋ subpopulation in CD56^＋ NK cells was 92. 1%, and among CD56^＋ CD226^＋ cells, CD56^bright CD226^＋ cells constituted the majority（89. 9%）. Our conclusions are that CD226 may be a differentiation marker on CD56^bright NK subset which was the very early appearing and predominant subpopulation of NK cells during the reconstitution of immune system by autologous peripheral blood hematopoietic stem cell transplantation.

关 键 词：CD226 NK细胞 亚群 干细胞移植 

分 类 号：R392[医药卫生—免疫学]