IL-18与急性移植物抗宿主病的关系  

Relationship between IL-18 and acute graft-versus-host disease

作　　者：徐敏[1] 丁慧芳[1] 耿丽[1] 路希敬[1] 

机构地区：[1]胜利油田中心医院血液内科,山东东营257034

出　　处：《免疫学杂志》2005年第6期504-506,共3页Immunological Journal

摘　　要：目的探讨白细胞介素18(IL18)与异基因小鼠骨髓移植后急性移植物抗宿主病(aGVHD)的关系及作用机制。方法C57BL/6小鼠随机分为A、B、C3组,经60Co照射后,A、B组经尾静脉移植入Balb/c小鼠骨髓细胞,C组移植入C57BL/6小鼠骨髓细胞。移植后,A组注射IL18,B、C组注射NS。测定B、C2组小鼠血清IL18浓度;比较A、B2组小鼠aGVHD的程度,酶联免疫法(ELISA)测定血清中干扰素γ(IFNγ)及白细胞介素10(IL10)的浓度。结果移植后B组小鼠血清IL18的浓度明显高于C组;A组小鼠较B组小鼠的aGVHD严重,血清IFNγ的浓度高,血清IL10的浓度低。结论①aGVHD发生时,血清IL18浓度升高。②IL18可以加重异基因骨髓移植后aGVHD反应。③IL18促进CD4+T细胞向CD4+Th1细胞分化可能是IL18加重aGVHD反应的机制之一。Objective To investigate the role IL-18 in acute graft-versus-host disease （aGVHD） after allogeneie bone marrow transplantation （Allo-BMT） in mice. Methods C57BL/6 recipient mice irradiated with ^60Co were randomly divided into A, B, and C groups. Mice in group A and B were transfused with Balb/c donor cells while mice in group C with C57BL/6 donor cells. After transplantation, recipient mice in group A were treated subcutaneously with 1μg IL-18 daily from day 0 to day 5 while recipient mice in group B and C with NS, separately. In group A and B, the severity of aGVHD was evaluated. Concentrations of IL-18, IFN-γ and IL-10 in serum of mice were measured by enzyme-linked immunoserbent assay （EHSA）. Results The concentration of IL-18 in serum of B group was higher than that of C group after transplantation, aGVHD response was more severe in group A than that in group B. The serum concentration of IFN-γ in group A was higher than that in group B, but the serum concentration of IL-10 was lower than that in group B. Conclusion The serum concentration of IL-18 is increased when aGVHD occurred in recipient mice after allogeneic transplantation. IL-18 may aggravate aGVHD response. IL-18 can promote the differentiation of CD4^＋ T cells into CD4^＋ Th1 cells, which may be one of the mechanisms of aggravating aGVHD.

关 键 词：IL-18 Γ干扰素 IL-10 急性移植物抗宿主病 小鼠 

分 类 号：R55[医药卫生—血液循环系统疾病]