填料粒径对制备色谱生产的影响  

Effect of Packing Material Size on Production of Preparative Chromatography

作　　者：周卫红[1] 郭威[1] 林炳昌[1] 

机构地区：[1]鞍山科技大学分离技术中心,辽宁鞍山114044

出　　处：《精细化工》2005年第11期831-834,847,共5页Fine Chemicals

摘　　要：随着绿色精细化工工业的发展,对制备色谱和模拟移动床色谱(SMBC)的需求越来越大.填料粒径的选择是制备色谱和模拟移动床色谱分离中的关键问题.填料粒径主要从柱效和压力两方面影响着制备色谱的应用.压力影响着运行成本,柱效影响着分离效果,但两者又互相制约.该文利用板高曲线和压力变化曲线斜率积对填料性能进行了比较.斜率积消除了填料粒径的影响,却又联系着其他与柱压力和柱效相关的因素.利用斜率积来评价填料性能对问题的分析更加深入.该文通过对不同粒径填料所填充柱的孔隙率、柱压和柱效的比较发现,随填料粒径减小,柱压力的上升幅度小于随填料粒径平方反比例增大的幅度,理论塔板高度减小.从银杏黄酮的模拟移动床色谱分离结果可以看出,使用粒径为20～25 μm的填料,其产品纯度低于使用粒径为30～40 μm的填料.Along with the development of green fine chemical industry, the demand for preparative chromatography and simulated moving bed chromatography（SMBC） is becoming larger and larger. The choice of packing material size is the key problem in separation with preparative chromatography and simulated moving bed chromatography. Effect of packing material size is mainly on the column pressure and column efficiency. Column pressure affects the running cost, column efficiency affects the separation degree, but they restrict each other. Performances of different packing materials were compared through the product of the slope of HETP（ height equivalent to a theoretical plate） curve and pressure curve. There is no item of packing material size in this product, but it relates to the correlative factors of column pressure and column efficiency, and thus deepens the analysis of problem. Column porosity, column pressure and column efficiency for different packing material size were compared. The results show that, with the decrease of packing material size, amplitude of the increase of column pressure is smaller than the amplitude of the increase of inverse square of packing material size, and the HETP decreases. The purity of ginkgo flavonoids from SMBC with 20 - 25 μm packing material is lower than that from SMBC with 30 -40μm packing material.

关 键 词：制备色谱 模拟移动床色谱 填料粒径 斜率积 银杏黄酮 

分 类 号：TQ450.43[化学工程—农药化工]