三氯化铬对2型糖尿病患者红细胞胰岛素受体的影响  

Influence of chromium trichloride on erythrocytic insulin receptor of patients with type 2 diabetes mellitus

作　　者：孔令芳[1] 钱聪[2] 李强[1] 侯永生[1] 刘国良[1] 

机构地区：[1]中国医科大学附属第一医院内分泌科,辽宁沈阳110001 [2]中国医科大学流行病学教研室,辽宁沈阳110001

出　　处：《中国现代医学杂志》2005年第20期3089-3092,共4页China Journal of Modern Medicine

摘　　要：目的探讨三价铬改善糖代谢的作用机制。方法以血铬正常的健康人作对照,将63例血铬降低的T2DM患者的红细胞悬液与不同浓度的三氯化铬进行体外孵育,测定孵育前、后碘标INS与红细胞INS受体结合物的放射性,并进行Scatchard分析。结果T2DM组较对照组血铬显著降低(P<0.01);碘标INS与受体的最大特异性结合率及r1、r2均降低(P<0.01),且空腹INS水平与受体的结合位点之间呈显著的负相关(r=-0.91,P<0.05)。加入三氯化铬后,与“0”浓度组比较,“100”浓度组仅r1增加(P<0.01),而“200”及“400”浓度组的最大特异性结合率及r1、r2均增加,k1及k2降低,均有统计学差异。结论三价铬可能通过增加受体的敏感性而增加INS与受体的结合率,从而改善IR调节糖代谢。[Objective] To explore the action mechanism of trivalent chromium improving glycometabohsm. [Methods] With normal serum chromium objects as controls, erythrocytic suspensions from 63 cases with type 2 diabetes and lower serum chromium were incubated in vitro with different concentrations of chromium trichloride. Radioactivity of iodine labeled insulin and erythrocytic insulin receptor conjugate before and after s/he incubation was measured and Scatchard analysis was done. [Results] Compared with controls, serum chromium, maximum specific combining rate between iodine labeled insulin and receptor, and high and low affinity receptor combining sites （r1 and r2） were reduced in type 2 diabetes group （P 〈0.01）. In type 2 diabetes group the correlation between fasting insulin level and receptor combining sites presented obviously negative correlation （r =-0.91, P 〈0.05）. After adding chromium trichloride, compared with 0 concentration group only r1 was increased in 100 concentration group （P 〈 0.01）. In 200 and 400 concentration groups maximum specific combining rates, r1 and r2 were increased, but two receptor affinity constants （k1 and k2） were reduced （P 〈0.05）. [Conclusion] Trivalent chromium may increase the combining rate between insulin and receptor through enhancing receptor＇ s sensitivity so as to improve insulin resistance and to regulate glycometabelism.

关 键 词：铬 2型糖尿病 红细胞胰岛素受体 胰岛素抵抗 

分 类 号：Q587.1[生物学—生物化学]