硫芥中毒复合烫伤早期大鼠血浆MDA和SOD的变化  

Early changes of blood MDA and SOD levels in rats with complex injury of MS intoxication and scalding

作　　者：蔡颖[1] 林海[1] 高钰琪[2] 杨和平[3] 袁发焕[4] 董兆君[1] 

机构地区：[1]第三军医大学预防医学系军事毒理学教研室,重庆400038 [2]第三军医大学高原医学系,重庆400038 [3]第三军医大学附属西南医院呼吸科,重庆400038 [4]第三军医大学新桥医院肾内科,重庆400038

出　　处：《疾病控制杂志》2005年第5期422-424,共3页Chinese Journal of Disease Control and Prevention

摘　　要：目的探讨硫芥中毒合并烫伤对大鼠血浆丙二醛和过氧化物岐化酶的影响。方法分别注射0.2LD50、0.4LD50、0.6LD50剂量的硫芥,以测定剂量-效应关系。大鼠以92℃热水致伤18s,建立硫芥中毒合并大鼠深Ⅱ度烫伤模型,同时注射硫芥0.6LD50。伤后1h、2h、6h、12h、24h取心脏血,常规方法测定血浆丙二醛含量和过氧化物岐化酶活性。结果单纯硫芥中毒和单纯烫伤均可引起丙二醛含量升高,6h开始下降。伤后2h丙二醛含量随硫芥剂量的增大而增加。过氧化物岐化酶活性在接触硫芥和烫伤后均显著降低。硫芥-烫伤复合伤动物血浆丙二醛含量和过氧化物岐化酶活性变化比单纯损伤强烈。结论硫芥中毒复合烫伤导致的脂质过氧化反应比单纯中毒或单纯烫伤严重。Objective To investigate the changes of blood maleic dialdehyde （MDA） concentration and superoxide dismutase （SOD） activity in rats treated with sulfur mustard, bum or sulfur mustard plus burn. Methods Wistar rats were anesthetized and a 30 % burn of total surface of the body was made by exposure of 92℃ water for 18. Sulfurmustard was injected. Results Both sulfurmustard injection and burn significantly increased blood MDA concentration, which reached the peak values at 2 h time point and began to recover at the 6 h time point. However, SOD activity decreased by sulfurmustard administration or burn. MDA concentration and SOD activity were further changed in the animals treated by a complex injury of sulfurmustard injection plus burn. Conclusions The results suggest that a complex injury of mustard intoxication plus burn may produce a more severe lipid peroxidation than those induced bv mustard treatment or burn onlv.

关 键 词：芥子气 烧伤 丙二醇 超氧化物岐化酶 

分 类 号：R595[医药卫生—内科学] R845.22[医药卫生—临床医学]