被动吸烟对大鼠骨代谢和血浆TNF水平的影响  

Effects of passive smoking on bone metabolism and plasma TNF level in rats

作　　者：雷光华[1] 李康华[1] 李晶[2] 周江南[1] 

机构地区：[1]中南大学湘雅医院骨科,长沙410008 [2]中南大学湘雅二医院脊柱外科

出　　处：《中国骨质疏松杂志》2005年第3期314-316,共3页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金资助项目(30300396)

摘　　要：目的探讨吸烟诱导骨吸收的可能机理。方法将32只Wister大白鼠随机分成4组,分别予以不吸烟、被动吸烟2个月、3个月和4个月;检测血清骨钙素、尿钙与尿肌酐以及血浆肿瘤坏死因子(TNF)水平。结果4组大鼠血清骨钙素水平差异无显著性;吸烟2个月组与对照组尿CaCr差异无显著性,吸烟3个月和4个月组大鼠尿CaCr分别为0.787±0.226和0.781±0.217,显著低于对照组(P<0.05)。3个实验组大鼠血浆TNF水平均显著高于空白对照组(P<0.05,P<0.01,P<0.01),且随吸烟时间延长TNF水平升高,二者呈正相关;直线回归方程式为Y=0.048X+0.8902,r=0.989(P<0.05)。结论被动吸烟可使大鼠骨吸收增强,从而导致骨丢失、发生骨质疏松;而吸烟升高血浆TNF水平可能是吸烟刺激骨吸收、导致骨质疏松的机理之一。Objective To investigate the pathogenesis of bone resorption induced by smoking. Methods Thirty- two Wister rats were distributed randomly into 4 groups ： A, B, C and D. Group A was used as sham control. Group B, C and D were exposed to passive cigarette smoking daily for 2,3 and 4 months, respectively. The level of plasma TNF was measured in the end. Correlation analysis was performed between TNF and the duration of smoking. The bone mineral density （BMD） in vivo of each group and the one of skeletons in vitro of group A and D was measured, and serum osteocalcin, urinary calcium, urinary creatinine were detected. Results The level of plasma TNF in group B, C and D were significantly greater than that of control and positively correlated to the duration of smoking（ Y = 0.048 X ＋ 0. 8902, r = 0.989, P 〈 0.05 ）. No difference was observed in serum osteocalcin among all gruups.The proportionate of urinary calcium/creatinine was significantly higher in group C and D （0.787 ± 0.226 and 0.781 ± 0.217） than that of control（ P 〈 0.05）. There was a negative correlation between BMD and the proportionate urinary calcium/creatinine in group D. Conclusions Passive smoking accelerates bone resorption, and increases bone loss leading to osteoporosis. This might due to the elevated serum TNF level caused by smoking.

关 键 词：骨质疏松 被动吸烟 肿瘤坏死因子 骨钙素 钙 肌酐 大鼠 TNF水平 血浆TNF 被动吸烟 大鼠尿 肿瘤坏死因子(TNF) 骨代谢 血清骨钙素 CA/CR WISTER 

分 类 号：R581.1[医药卫生—内分泌] R743.33[医药卫生—内科学]