Capillary Electrophoretic Determination of Jinggangmycin A in Formulations Using Direct UV Detection  

作　　者：Jin HE Xin LIU Yi Shi LIU Zi Niu YU 

机构地区：[1]National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070

出　　处：《Chinese Chemical Letters》2005年第11期1511-1514,共4页中国化学快报（英文版）

摘　　要：A simple, fast and reliable method was developed for the analysis of jinggangmycin A （validamycin A） in commercial formulations. The running buffer used was acetate buffer （100 mmol/L, pH 4.7） with 15 kV as the applied voltage. The detection was achieved by using direct UV mode at 200 nm and the detection limit was 0.2 μg/mL. Linearity in the concentration range of 5-500 μg/mL was excellent （RE 〉 0.999）. The run-to-run repeatability （n = 3）, as expressed by the relative standard deviation （RSD） for migration times and peak areas were less than 0.5% and 3.0% respectively. The mean recovery ranged from 97.2% to 101.4%.A simple, fast and reliable method was developed for the analysis of jinggangmycin A （validamycin A） in commercial formulations. The running buffer used was acetate buffer （100 mmol/L, pH 4.7） with 15 kV as the applied voltage. The detection was achieved by using direct UV mode at 200 nm and the detection limit was 0.2 μg/mL. Linearity in the concentration range of 5-500 μg/mL was excellent （RE 〉 0.999）. The run-to-run repeatability （n = 3）, as expressed by the relative standard deviation （RSD） for migration times and peak areas were less than 0.5% and 3.0% respectively. The mean recovery ranged from 97.2% to 101.4%.

关 键 词：Jinggangmycin A validamycin A capillary zone electrophoresis direct UV detection. 

分 类 号：O646.2[理学—物理化学]