Enzymatic Glycerolysis of Chinese Vegetable Tallow Fraction by Lipase and Study of the Mechanism  

脂肪酶促乌桕脂组分甘油解及其反应机理研究

作　　者：尹春华 刘涛 谭天伟 

机构地区：[1]Beijing University of Chemical Technology

出　　处：《Chinese Journal of Chemical Engineering》2005年第5期656-662,共7页中国化学工程学报（英文版）

基　　金：国家重点基础研究发展计划(973计划)，国家自然科学基金，the National Key Technology Program，国家高技术研究发展计划(863计划)

摘　　要：Glycerolysis of Chinese vegetable tallow (CVT) fraction was investigated using a 1,3-specific lipase from Rhizopus arrhizus as catalyst. Based upon a binary gradient HPLC with an evaporative light-scattering detector (ELSD), the contents of free fatty acids (FFA), monoglycerides (MG), diglycerides(DG) and triglycerides (TG)with their positional isomers during the glycerolysis were determined. The effects of water content and the ratio of glycerol to oil on the product distribution of glycerolysis were studied. Under the optimum reactant conditions:250 units lipase per gram oil at 37 ℃ with 1:2 molar ratio of oil to glycerol in a solvent-free system, after 24 h reaction, the product consisted of 7.2% TG, 25.6% MG, 56.1% DG and 4.9% FFA (all by mass). Furthermore, the mechanism of glycerolysis was discussed in detail.Glycerolysis of Chinese vegetable tallow （CVT） fraction was investigated using a 1,3-specific lipase from Rhizopus arrhizus as catalyst. Based upon a binary gradient HPLC with an evaporative light-scattering detector （ELSD）, the contents of free fatty acids （FFA）, monoglycerides （MG）, diglycerides（DG） and triglycerides （TG） with their positional isomers during the glycerolysis were determined. The effects of water content and the ratio of glycerol to oil on the product distribution of glycerolysis were studied. Under the optimum reactant conditions： 250 units lipase per gram oil at 37℃ with 1：2 molar ratio of oil to glycerol in a solvent-free system, after 24 h reaction, the product consisted of 7.2% TG, 25.6% MG, 56.1% DG and 4.9% FFA （all by mass）. Furthermore, the mechanism of glycerolysis was discussed in detail.

关 键 词：GLYCEROLYSIS Rhizopus arrhizus LIPASE Chinese vegetable tallow 

分 类 号：Q54[生物学—生物化学] Q55