Determination of hemoglobin adduct of a musk xylene metabolite in trout as biomarker of exposure by gas chromatography mass spectrometry  

作　　者：Mohammad Abdul KARIM Mohammad Rezaul 

机构地区：[1]Department of Chemistry and Biochemistry, Baylor University, P.O. Box 97348, Waco, TX 76798-7348, USA,Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh

出　　处：《Journal of Environmental Sciences》2005年第6期989-992,共4页环境科学学报（英文版）

摘　　要：Musk xylene（MX） is frequently used as fragrances in formulation of personal care products. Quantification of a bound 4-amino- MX（4-AMX） as cysteine adducts in trout hemoglobin（Hb） was made by gas chromatography-ion trap-mass spectrometry（GC/MS）. The Hb samples were collected from trout after 24 h exposure to MX at 10μg/g, and or menhaden oil（control）. The formation of cysteine-Hb adduct was observed from nitroso derivative of MX, released by alkaline hydrolysis. The released 4-AMX metabolite was extracted in n- hexane. The extract was then reduced by evaporation, and analyzed by GC/MS. When similar agreement of mass spectral features and retention time of 4-AMX were obtained in both standard and sample solutions, the presence of 4-AMX metabolite in the Hb was confirmed. The concentration of 4-AMX was found to be 3.1 × 10^-6 6.9 × 10^-6 mg/g in the Hb solution. Quantitation was made based on an internal standard, a calibration plot, and response factor. In the non-hydrolyzed and laboratory blank extracts, the 4-AMX metabolite was not detected. Additionally, coeluting and interfefing ions were observed in the biological samples.Musk xylene（MX） is frequently used as fragrances in formulation of personal care products. Quantification of a bound 4-amino- MX（4-AMX） as cysteine adducts in trout hemoglobin（Hb） was made by gas chromatography-ion trap-mass spectrometry（GC/MS）. The Hb samples were collected from trout after 24 h exposure to MX at 10μg/g, and or menhaden oil（control）. The formation of cysteine-Hb adduct was observed from nitroso derivative of MX, released by alkaline hydrolysis. The released 4-AMX metabolite was extracted in n- hexane. The extract was then reduced by evaporation, and analyzed by GC/MS. When similar agreement of mass spectral features and retention time of 4-AMX were obtained in both standard and sample solutions, the presence of 4-AMX metabolite in the Hb was confirmed. The concentration of 4-AMX was found to be 3.1 × 10^-6 6.9 × 10^-6 mg/g in the Hb solution. Quantitation was made based on an internal standard, a calibration plot, and response factor. In the non-hydrolyzed and laboratory blank extracts, the 4-AMX metabolite was not detected. Additionally, coeluting and interfefing ions were observed in the biological samples.

关 键 词：biomarkers musk xylene metabolite Hb adducts fish exposure 

分 类 号：X51[环境科学与工程—环境工程]