Distribution characteristics of ammonia-oxidizing bacteria in the Typha latifolia constructed wetlands using fluorescent in situ hybridization(FISH)  

作　　者：YAN Li Ryuhei Inamori GUI Ping XU Kai-qin KONG Hai-nan Masatoshi Matsurnura Yuhei Inamori 

机构地区：[1]School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China [2]Institute of Applied Biochemistry, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-0006, Japan [3]National Institute for Environmental Studies, 16-2, Onogawa, Tsukuba, Ibaraki 305-8506, Japan

出　　处：《Journal of Environmental Sciences》2005年第6期993-997,共5页环境科学学报（英文版）

基　　金：TheHiTechResearchandDevelopmentProgram(863)ofChina(No.2002AA601013)

摘　　要：A molecular biology method, fluorescent in situ hybridization（FISH）, in which the pre-treatment was improved in allusion to the media of the constructed wetlands（CW）, e.g. the soil and the grit, was used to investigate the vertical distribution characteristics of ammonia-oxidizing bacteria（AOB） quantity and the relation with oxidation-reduction potential（ORP） in the Typha latifolia constructed wetlands under three different Ioadings in summer from May to September. Results showed that the quantity of the AOB decreased in the Typha latifolia CW with the increase of vertical depth. However, the AOB quantity was 2-4 times the quantity of the control in the root area. Additionally, ORP in the rhizosphere was found to be higher than other areas, which showed that Typha latifolia CW was in an aerobic state in summer when using simulated non-point sewage at the rural area of Taihu Lake in China and small town combined sewage.A molecular biology method, fluorescent in situ hybridization（FISH）, in which the pre-treatment was improved in allusion to the media of the constructed wetlands（CW）, e.g. the soil and the grit, was used to investigate the vertical distribution characteristics of ammonia-oxidizing bacteria（AOB） quantity and the relation with oxidation-reduction potential（ORP） in the Typha latifolia constructed wetlands under three different Ioadings in summer from May to September. Results showed that the quantity of the AOB decreased in the Typha latifolia CW with the increase of vertical depth. However, the AOB quantity was 2-4 times the quantity of the control in the root area. Additionally, ORP in the rhizosphere was found to be higher than other areas, which showed that Typha latifolia CW was in an aerobic state in summer when using simulated non-point sewage at the rural area of Taihu Lake in China and small town combined sewage.

关 键 词：constructed wetland（CW） fluorescent in situ hybridization（FISH） ammonia-oxidizing bacteria （AOB） Typha/atifo/ia（cattail） 

分 类 号：X524[环境科学与工程—环境工程]