Biological activity of a red-tide alga—A. tamarense under co-cultured condition with bacteria  

作　　者：SU Jian-qiang YU Zhi-ming TIAN Yun SONG Xiu-xian HONG Hua-sheng ZHENG Tian-ling 

机构地区：[1]School of Life Sciences, Xiamen University, Xiamen 361005, China [2]State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China [3]Key Laboratory of Marine Ecology and Environmental Science, ChineseAcademy of Sciences, Qingdao 266071, China

出　　处：《Journal of Environmental Sciences》2005年第6期1047-1050,共4页环境科学学报（英文版）

基　　金：TheNationalBasicResearchProject(973)ofChina(No.2001CB409710)andtheNationalNaturalScienceFoundationofChina(No.30370276)

摘　　要：The relationship between Alexandrium tamarense （Lebour） Balech, one of red-tide alga, and two strains of marine bacteria, Bacillius megaterium（S7 ） and B. halmapulus（ S10 ） isolated from Xiamen Western Sea, was investigated by evaluating the growth state of A. tamarense and the variation of β-glucosidase activity in co-culture system. The results showed the growth and multiplication of the alga were related with the concentration, genus speciality of the bacteria, and growth stage of the alga itself. The growth of A. tamarense was obviously inhibited by S7 and S10 at high concentration. Either inhibition or promotion contributed much more clearly in earlier than in later stageof the growth of the alga. Furthermore, there was a roughly similar variation trend of the activity of extra-cellular enzyme, β- glucosidase, in the water of the separately co-cultured bacteria S7 and S10 with the alga. The β-glucosidase activity（β-GlcA） rapidly increased during the later algal growth accompanying the increase of the lysis of the alga cells. The obvious inhibition of A. tamarense by marine bacteria at high concentration and evident increase of β-GlcA in co-colture system would help us in better understanding the relationship between red-tide alga and bacteria, and also enlightened us the possible use of bacteria in the bio-control of red-tide.The relationship between Alexandrium tamarense （Lebour） Balech, one of red-tide alga, and two strains of marine bacteria, Bacillius megaterium（S7 ） and B. halmapulus（ S10 ） isolated from Xiamen Western Sea, was investigated by evaluating the growth state of A. tamarense and the variation of β-glucosidase activity in co-culture system. The results showed the growth and multiplication of the alga were related with the concentration, genus speciality of the bacteria, and growth stage of the alga itself. The growth of A. tamarense was obviously inhibited by S7 and S10 at high concentration. Either inhibition or promotion contributed much more clearly in earlier than in later stageof the growth of the alga. Furthermore, there was a roughly similar variation trend of the activity of extra-cellular enzyme, β- glucosidase, in the water of the separately co-cultured bacteria S7 and S10 with the alga. The β-glucosidase activity（β-GlcA） rapidly increased during the later algal growth accompanying the increase of the lysis of the alga cells. The obvious inhibition of A. tamarense by marine bacteria at high concentration and evident increase of β-GlcA in co-colture system would help us in better understanding the relationship between red-tide alga and bacteria, and also enlightened us the possible use of bacteria in the bio-control of red-tide.

关 键 词：Alexandrium tamarense Β-GLUCOSIDASE BACTERIA 

分 类 号：X55[环境科学与工程—环境工程]