高效液相色谱法测定舒泌通胶囊中绿原酸的含量  

Determination of Chlorogenic Acid in Shubitong Capsules by HPLC

作　　者：李哲媛[1] 孟芹[1] 范兵[1] 向金莲[1] 

机构地区：[1]云南省药品检验所,昆明650011

出　　处：《中国药师》2005年第11期917-919,共3页China Pharmacist

摘　　要：目的:建立舒泌通胶囊中绿原酸的高效液相色谱法。方法:采用C18柱,以甲醇-水-冰醋酸-三乙胺(15:85:1:0．3)为流动相,流速1．0 ml·min-1检测波长328 nm,外标法定量。结果:绿原酸含量测定线性范围为5-4 840 ng(r=1．000 0),平均回收率为100.7％,RSD为1．16％(n:6)。结论:高效液相色谱法专属、灵敏、重现性好,可用于该制剂的质量控制。Objective： To establish a HPLC method for determination the content of chlorogenic acid in Shubitong capsules. Method： The C18 column was used. The mobile phase was methanol-water-acetic acid-triethylamine （15： 85： 1：0.3）. The detection wavelength was 328 nm. The external standard method was used. Result： Chlorogenic acid showed a good linearity within the range of 5 -4 840 ng. The average recovery was 100.7% （RSD = 1.16% ）. Conclusion： The method is simple, accurate and reproducible. It can be used for the quality control of Shubitong capsules.

关 键 词：高效液相色谱法 舒泌通胶囊 绿原酸 

分 类 号：R927.2[医药卫生—药学]