高效液相色谱法测定甲硫氨酸维生素B_1注射液中甲硫氨酸和维生素B_1的含量  

Determination of Methionine and Vitamin B_1 in Injection of Methionine and Vitamin B_1 by HPLC

作　　者：黄榕珍[1] 

机构地区：[1]福建省药品检验所,福州350001

出　　处：《海峡药学》2005年第5期42-45,共4页Strait Pharmaceutical Journal

摘　　要：目的建立一种快速,准确的高效液相色谱法同时测定甲硫氨酸维生素B1注射液中甲硫氨酸和维生素B1的含量.方法采用C18柱 5μm(4.6 ×250mm),流动相为乙腈–0.5mol·L-1庚烷磺酸钠(20 :80),检测波长为25nm.结果甲硫氨酸的线性范围是162.1～648.3μg·mL-1,r=0.9999,平均回收率为99.83%, RSD=0.485;维生素B1的线性范围是16.96～67.84μg·mL-1, r=0.9998,平均回收率为 99.17%,RSD=1.42.OBJECTIVE A HPLC method for the determination of Determination of Methionine and Vitamin B1 in Injection of Methionine and Vitamin B1 was established. METHODS A C18 column 5μm （4. 6×250mm） was used with Acetonitrile Sodium 1-Heptanesulfonate 0. 5mol· L^-1 （20： 80） as mobile phase. The detection wave length was 225nm. RESULTS The linearity range and average recovery rate for Merhionine were 162.1-648. 3μg· mL^-1, r=0. 9999, 99.83% and RSD=0. 485. while those for Vit B, were 16. 96-67.84μg· mL^-1, r=0. 9998, 99.17% and RSD=1.42. CONCLUSION The method couldbe used to determine the content of Methionine and Vitamin B1 in Injection of Methionine and Vitamin B1 rapidly and acourately.

关 键 词：甲硫氨酸 维生素B1 高效液相色谱法 含量测定 

分 类 号：R927.4[医药卫生—药学]