小麦雌性不育基因的SSR多态性标记  

Research of the Sterile Gene for Female Sterile Wheat by Microsatellite Marker

作　　者：李生强[1] 窦秉德[1] 侯北伟[2] 许盛宝[1] 曹俊梅[1] 陈莉[1] 

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]淮阴师范学院生物系,江苏淮安223300

出　　处：《新疆农业大学学报》2005年第3期9-12,共4页Journal of Xinjiang Agricultural University

基　　金：国家自然科学基金项目(30060044);淮阴师范学院博士基金资助项目

摘　　要：以普通小麦新601×雌性不育小麦XND126的F2群体作为育性调查以及基因标记群体。通过对育性基因的分析,确定在此组合中雌性不育基因表现为1对主效基因控制;结合混合分组分析法(Bulk Segregant Analy-sis,BSA),首次对小麦雌性不育基因进行了SSR标记研究,通过对一千对微卫星引物的筛选,初步确定了微卫星引物cfd36和gwm296与主效基因连锁。A study was carried out through the genetic analysis of F2 population which is derived from a common wheat （new 601）and the female sterile line （XND126）. The result indicated that the sterile gene was performed by a pair recessive gene in the cross. The F2 population was used to mark the female sterile gene by microsatellite and BSA （bulked segregant analysis） at the first time. Among the 1,000 pairs of microsatellite primers, two markers were found to be polymorphic between the two pools and the two parents. The experimental research showed that microsatellite marker cfd36 and gwm296 were linked with the major sterile gene.

关 键 词：小麦 雌性不育基因 SSR 

分 类 号：Q343.12[生物学—遗传学]