RP-HPLC法测定定坤丸中芍药苷的含量  

Determination of the content of paeoniflorin in Dingkun pills by RP-HPLC

作　　者：王莉[1] 崔明[1] 文艳秋 徐新盛 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]北京中丰天恒医药技术开发有限公司,北京100012

出　　处：《西北药学杂志》2005年第5期202-203,共2页Northwest Pharmaceutical Journal

摘　　要：目的建立定坤丸中芍药苷含量的测定方法.方法采用反相高效液相色谱法,Kromasil C18柱(4.6 mm×250 mm,5μm),以乙腈-水梯度系统为流动相,检测波长230 nm.结果芍药苷在3.664～36.64 mg·L-1范围内线性关系良好,r=0.999 7(n=5);平均回收率为99.6%(n=9),RSD为1.0%.结论本法简便、准确,重现性好,为定坤丸的质量评价提供了依据.Objective A high-performance liquid chromatographic method was developed for the determination of the content of paeoniflorin in Dingkun pills. Method A Kromasil C18 column（4.6 mm × 250 mm ,5 μm） was used, with the mobile phase consisting of acetonitrile-H2O binary system. The detection wavelength was 230 nm. Results The linear range of paeoniflorin was 3. 664-36.64 mg·L^-1, r = 0. 999 7（ n = 5） ; the average recovery was 99.6 % （ n = 9）, RSD was 1.0 %. Conclusion The method is simple and can be used to determine paeoniflorin in Dingkun pills with satisfactory accuracy and reproducibility.

关 键 词：定坤丸 芍药苷 反相高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]