高效液相色谱法测定胰炎灵颗粒中大黄素的含量  

Determination of Emodin in Yiyanlin Granule by RP-HPLC

作　　者：周琴妹[1] 邵家德[1] 陈晓斌[1] 朱政中[1] 

机构地区：[1]江苏省中医院,江苏南京210029

出　　处：《中国中医药信息杂志》2005年第10期47-47,51,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立胰炎灵颗粒定量标准。方法采用高效液相色谱法,DisveryC18柱(150mm×4.6mm,5μm);流动相:甲醇-水(75∶25);流速:1mL/min;检测波长254nm;柱温:40℃。结果大黄素在1.4460～7.2300μg间呈良好的线性关系,r=0.9997,加样回收率为98.71%,RSD=2.12%。结论该法具有操作简单、准确的优点,分离效果显著。Objective To develop a sensitive and specific HPLC assay for determination of emodin in Yiyanlin granule, Methods Separation was performed on a Disvery C18 column while the thermostat was set at 40 ℃. The mobile phase consists of methol-water （75 ： 25）. The flow rate was 1.0 mL/min, The UV detection wavelength was set at 254 nm. Results The relationship between response and the concentration of emodin was linear well in the range of 1.4460-7.230 μg r=0.9997, the average recovery was 98.71%, and the RSD was 2.12%. Conclusion The HPLC method was simple, sensitive and specific. It can be applied to the determination of emodin in this preparation.

关 键 词：胰炎灵颗粒 大黄素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]