低密度、氧化低密度脂蛋白对内皮细胞分泌内皮素的影响  

Effect of low density lipoprotein and oxidized low density lipoprotein on the secretion of endothelin in endothelial cells

作　　者：张敏[1] 胥学伟[2] 李小鹰[3] 

机构地区：[1]解放军205医院神经消化科,辽宁锦州市121000 [2]解放军205医院内四科,辽宁锦州市121000 [3]解放军总医院南六科,北京市100853

出　　处：《中国康复理论与实践》2005年第10期785-786,共2页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的观察低密度脂蛋白(LDL)、氧化低密度脂蛋白(ox-LDL)对内皮细胞分泌内皮素(ET)的影响,以及后者在体外是否对前者具有氧化修饰作用。方法采用不同浓度的LDL、ox-LDL及LDL+ox-LDL与脐静脉内皮细胞株ECV-304进行孵育,24 h后分别收集细胞和上清液,采用放免分析法测定ET含量。结果LDL、ox-LDL均能促进内皮细胞合成、分泌ET,但ox-LDL的作用更显著,而LDL+ox-LDL组上清ET浓度大于两者单独作用之和。结论LDL、ox-LDL均能促进内皮细胞合成、分泌ET,但后者的作用更为明显,且在体外对LDL具有氧化修饰作用。Objective To investigate the effect of low density lipoprotein （LDL） and oxidized low density lipoprotein （ox-LDL） on the secretion of endothelin （ET） and whether LDL to be oxidized and modified by ox-LDL in vitro. Methods Endothelial cell strain ECV-304 was incubated with different concentration of LDL （50 and 100 mg/L）, ox-LDL （50 and 100 mg/L） or LDL-ox-LDL （50mg/L） for 24 hours, then cells and cultured medium were collected. ET in cells and cultured medium were detected with radioimmunoassay. Results Both of LDI. and ox-LDL enhanced the secretion of ET in cultured endothelial cells; the effect of ox-LDL was more markedly than LDL. LDL＋ox-LDL had a greater effect than any of the two lipoproteins alone. Conclusion LDI. and ox-LDL may have the ability to promote the secretion of ET in cultured endothelial cells and LDL can be oxidized and modified by ox-LDL in vitro.

关 键 词：脂蛋白 氧化低密度脂蛋白 内皮细胞 内皮素 

分 类 号：Q513[生物学—生物化学] R541.4[医药卫生—心血管疾病]