妊高征患者胎盘组织中瘦素mRNA表达与NO含量的测定及临床研究  

Clinical significance of leptin mRNA and nitric oxide concentration in placenta of patients with pregnancy-induced hypertension

作　　者：司红卫[1] 杜惠敏[1] 阎立清[1] 于新艳[1] 李建[1] 

机构地区：[1]泰安市中心医院妇产科,山东271000

出　　处：《中国优生与遗传杂志》2005年第10期34-36,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的检测正常妊娠、妊高征妇女胎盘组织中瘦素mRNA的表达及与一氧化氮含量的关系,探讨瘦素在妊高征发病中的作用。方法用逆转录-聚合酶链技术(RT-PCR)测定38例正常妊娠妇女、62例妊高征妇女分娩时胎盘组织中瘦素mRNA表达水平;用硝酸还原酶法测定胎盘组织NO的含量。结果(1)正常晚孕及妊高征妇女胎盘瘦素mRNA表达水平分别为:0.139±0.12、0.603±0.287(其中轻、中、重度妊高征胎盘瘦素水平分别为:0.145±0.056、0.38±0.122、0.75±0.199)。(2)妊高征组胎盘瘦素水平明显高于正常晚孕组,差异有显著性(P<0.01),妊高征患者胎盘瘦素水平随病情加重而升高;重、中、轻度之间差异均有显著性(P<0.01、P<0.05)。(3)妊高征胎盘组织中NO含量低于正常晚孕妇女,差异有显著性(P<0.05);重度妊高征患者胎盘NO含量明显低于中度、中度低于轻度,差异有显著性(P<0.01、P<0.05),(4)妊高征胎盘瘦素mR-NA表达与NO含量呈负相关(r=-0.6294,P<0.01),与收缩压、舒张压、平均动脉压、尿蛋白呈正相关(r=0.547,0.377,0.45,0.517;P=0.015,0.015,0.020,0.040),重度妊高征组与新生儿体重呈负相关(r=-0.447,P=0.018)。结论胎盘瘦素mRNA表达水平在妊高征组中明显升高,随着妊高征病情加重,胎盘瘦素mRNA表达逐渐增加;同时,胎盘组织中NO含量在妊高征妇女中明显降低,胎盘瘦素mRNA表达与胎盘组织中NO含量呈负相关。因此,瘦素可能与妊高征发病有关,可望成为妊高征病情发展的检测指标之一。Objective： To investigate the placental leptin mRNA in normal pregnancies and patients with PIH and to study its clinical significance. Methods： RT - PCR was used to measure the levels of leptin mRNA in 38 healthy normal the third trimester pregnant women and 62 patients with PIH （ 15 mild, 18 moderate and 29 severe）. Nitrite reduetase was used to measure the concentration of nitric oxide of placenta. Results： （1） The levels of leptin mRNA of placenta in normal pregnant women and PIH were 0. 139 ± 0.12,0. 603 ± 0. 287 respectively . （ mild, moderate and severe PIH ： 0. 145 ± 0.056,0.38 ± 0. 122,0.752 ± 0. 199 respectively. ） （2） Leptin mRNA levels in PIH were higher than those in normal pregnant women（ P 〈 0.01 ）, and there was great difference between mild , moderate and severe in PIH （ P 〈 0.05）. （3） The concentrations of placental NO in PIH group were lower than normal pregnant women（ P 〈 0.01 ）, and they were decreased gradually according to mild; moderate; severe of PIH （ P 〈 0.05, P 〈 0.01）. （4） Leptin mRNA levels of placenta in PIH negatively correlated with NO concentrations of placenta in PIH, （ r = - 0.6294, P 〈 0.01）. （5）The mRNA levels of placenta positively correlated with the levels of SBP, DBP, MAP and albuminuria （ r = 0.547,0.377,0.45,0.517 respectively）. Conclution： The resultes suggests that the levels of leptin mRNA in placenta are gradually increased , according to the mild, moderate and severe of PIH. Which might play an important role in pathogenesis of PIH. Elevated placenta leptin mRNA levels may be one of the monitor of PIH.

关 键 词：妊娠并发症 高血压 胎盘 瘦素mRNA NO 

分 类 号：R714.246[医药卫生—妇产科学]