山羊乳腺上皮细胞的光镜观察  

Observed on Goat Mammary Epithelial Cells by Light Microscope

作　　者：郑月茂[1] 安志兴[1] 石玉强[1] 张涌[1] 

机构地区：[1]西北农林科技大学生物工程研究所,陕西杨凌712100

出　　处：《中国农学通报》2005年第9期4-6,共3页Chinese Agricultural Science Bulletin

基　　金：国家科技部863高技术研究发展规划资助项目"家畜体细胞克隆技术体系的建立和应用"(2001AA213081)

摘　　要：采用电穿孔转化法,将EGFP(增强型绿色荧光蛋白)基因转化体外培养的山羊乳腺上皮细胞。细胞穿孔转化7d后在荧光显微镜下观察。结果显示,EGFP基因成功地转入体外培养的山羊乳腺上皮细胞,EGFP基因获得表达,6种电穿孔参数的转化效果基本相同。The present experiment aimed to transfect EGFP gene into goat mammary epithelial cells which culcured in vitro using electrotransfection. The cells were observed under fluoroscope when they were cultured over seven days after being transfected. The results were as follows： EGFP gene was successfully transfected into goat mammary epithelial cells and the expressing of EGFP gene were observed. The electrotransfection efficiency were same using six kinds of electrotransfection parameter.

关 键 词：山羊乳腺上皮细胞 电穿孔 EGFP基因 乳腺上皮细胞 光镜观察 山羊 绿色荧光蛋白 体外培养 基因转化 镜下观察 

分 类 号：S827.1[农业科学—畜牧学] S763.7[农业科学—畜牧兽医]