5种抗肿瘤抗生素对人白血病细胞DNA嵌合能力的观察  

Experimental observation of intercalating ability of fiveantitumor antibiotic on human leukemic cells DNA

作　　者：金晓明[1] 李奇志[1] 王小芹[1] 

机构地区：[1]福建省医学科学研究所

出　　处：《中国医院药学杂志》1996年第2期51-52,共2页Chinese Journal of Hospital Pharmacy

摘　　要：采用琼脂糖电泳法观察５种抗癌药对白血病细胞ＤＮＡ的嵌合能力。结果发现表阿霉素同进口阿霉素在５０μｇ／ｍｌ浓度时可与ＤＮＡ发生嵌合效应；国产阿霉素在较高浓度（１００μｇ／ｍｌ）和柔红霉素需高浓度（２００μｇ／ｍｌ）才显示嵌合效应；而米托蒽醌在较低浓度（２５μｇ／ｍｌ）即可发生此作用。本实验体现了作用机制类同的抗肿瘤药物对ＤＮＡ嵌合能力的差异。he intercalating ability of five antitumor drugs on human leukemic cells DNA was observed by agarose elec-trophoresis analysis. The experiment showed that epirubicin intercalated with the DNA at the drug concentration(50μg/ml) was similar to Italy-made doxorubicin. China-made doxorubicin intercalated with the DNA at higherconcentration (100μg/ml) ,daunorubicin at the highest concentration (200μg/ml) , whereas mitoxantrone intercalat-ed with the DNA at lower concentration (25μg/ml). The differences of intercalating ability of these antitumordrugs which are characterized by the same action mechanism on the DNA are realized in this experiment.

关 键 词：白血病细胞 DNA 嵌合效应 抗肿瘤抗生素 

分 类 号：R979.14[医药卫生—药品] R733.705[医药卫生—药学]