Cloning and Sequence Analysis of cDNA Encoding MRJP3 of Apis cerana cerana  

作　　者：SU Song-kun ZHNEG Huo-qing CHEN Sheng-lu ZHONG Bo-xiong Stefan Albert 

机构地区：[1]College of Animal Science, Zhejiang University, Hangzhou 310029, P.R.China [2]Institute of Medical Radiation and Cell Research, Würzburg 97078, Germany

出　　处：《Agricultural Sciences in China》2005年第9期707-713,共7页中国农业科学（英文版）

基　　金：the National Natural Science Foundation of China(30200206); Zhejiang Provincial Natural Science Foundation of China(302113).

摘　　要：By screening the worker （Apis cerana cerana） heads cDNA library using a fragment of the mrjp3 gene of Apis cerana as probe, 120 positive clones were obtained. The clone containing A. cerana cerana MRJP3 （AccMRJP3） cDNA was selected. Based on the sequencing of the inserts of the positive clone, a sequence of AccMRJP3 cDNA which is 1 887 bp long including a poly （A） tail was obtained. The AccMRJP3 cDNA encompassed an open-reading frame （ORF） with 1 779 bp encoding 593 amino acids. The un-translated regions （UTR） of the 5′ end and 3′end are 46 bp and 160 bp in length, respectively. Similar to AmMRJP3 and AdMRJP3, the putative AccMRJP3 also has a repetitive region. The comparison of the repetitive region of AccMRJP3, AmMRJP3 and AdMRJP3 shows some differences between them.By screening the worker （Apis cerana cerana） heads cDNA library using a fragment of the mrjp3 gene of Apis cerana as probe, 120 positive clones were obtained. The clone containing A. cerana cerana MRJP3 （AccMRJP3） cDNA was selected. Based on the sequencing of the inserts of the positive clone, a sequence of AccMRJP3 cDNA which is 1 887 bp long including a poly （A） tail was obtained. The AccMRJP3 cDNA encompassed an open-reading frame （ORF） with 1 779 bp encoding 593 amino acids. The un-translated regions （UTR） of the 5′ end and 3′end are 46 bp and 160 bp in length, respectively. Similar to AmMRJP3 and AdMRJP3, the putative AccMRJP3 also has a repetitive region. The comparison of the repetitive region of AccMRJP3, AmMRJP3 and AdMRJP3 shows some differences between them.

关 键 词：Apis cerana cerana MRJP3 Gene cloning Sequence analysis 

分 类 号：S891[农业科学—特种经济动物饲养]