中华眼镜蛇毒组分抑制肺癌细胞H1299的血管生长因子VEGF和Bfgf  

China cobra venom active factor (CCVAF) inhibits VEGF and bFGF in lung cancer cell line H1299

作　　者：余红娥[1] 余清声[1] 刘新艳[1] 朱柳[1] 楼晓华[2] 腾脉坤[2] 

机构地区：[1]广州医学院药物研究中心 [2]中国科学技术大学生命科学学院

出　　处：《中国临床药理学与治疗学》2005年第9期970-973,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：卫生部科技基金项目(№982349);广东省自然科学基金项目(№980463)

摘　　要：目的:了解中华眼镜蛇毒活性组分(CCVAF)对人非小细胞肺癌细胞株H1299VEGF、bFGF表达的抑制作用。方法:采用RTPCR方法检测H1299细胞中VEGF、bFGFmRNA表达的变化;利用ELISA检测培养上清中VEGF、bFGF蛋白的含量。结果:不同浓度的(2.0、4.0μg·ml-1)CCVAF对H1299bFGFmRNA水平有降低作用,其中4.0μg·ml-1CCVAF作用7h后bFGFmRNA的表达明显降低,但对VEGFmRNA的表达无明显影响。CCVAF作用H1299细胞24h后,其细胞上清中VEGF、bFGF的含量较对照组明显减少(P<0.05),且呈剂量依赖性。结论:CCVAF可抑制H1299细胞bFGFmRNA和蛋白的表达及VEGF蛋白的表达。AIM： To study the effect of China cobra venom active factor （CCVAF） on the expression of VEGF and bFGF in human non-small cell lung cancer cell line H1299. METHODS： VEGF and bFGF mRNA level was measured by RT-PCR. VEGF and bFGF protein level in the supematant was examined by ELISA. RESULTS： The mRNA expression level of bFGF was also reduced after treatment with the CCVAF in dose-dependent manner. Giving the CCVAF 4.0/μg·ml^-1 to cell H1299 for 4 hours could notablely decreased the mRNA expression of bFGF while it had no such effect on VEGF mRNA expression level. The content of VEGF, and bFGF in supematant of H1299 cells were significantly decreased after treatment for 24 hour with CCVAF in dose-dependent manner. There was significant difference compared with control group （P〈0.05）. CONCLUSION： CCVAF inhibits the expression of VEGF and bFGF in H1299 cells.

关 键 词：中华眼镜蛇毒 肺癌细胞 RT-PCR ELISA VEGF BFGF 

分 类 号：R979.1[医药卫生—药品]