黄芪提取物对大鼠全脑缺血再灌后炎症反应的影响  

Effects of extract of astragalus on inflammatory reaction during global cerebral ischemia and reperfusion in rats

作　　者：李静[1] 明亮[1] 黄茸茸[1] 曹曦[1] 吴强[2] 李卫平[1] 

机构地区：[1]安徽医科大学药理学教研室 [2]安徽医科大学病理学教研室

出　　处：《中国临床药理学与治疗学》2005年第9期1050-1052,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：安徽省十五科技重点专项(№01803016);安徽省自然科学基金(№00144414)

摘　　要：目的:探讨黄芪提取物对大鼠全脑缺血再灌后炎症反应的影响。方法:采用四血管阻塞法,复制大鼠全脑缺血再灌注模型;观察黄芪提取物对大鼠全脑缺血再灌后外周血白细胞和中性粒细胞计数、脑组织皮质和海马骨髓过氧化物酶(MPO)活性的影响;并对脑组织皮质做病理学检查。结果:与假手术组相比,模型组大鼠全脑缺血再灌注后外周血白细胞和中性粒细胞计数、脑组织皮质和海马MPO活性均明显升高;与模型组相比,黄芪提取物(20、40、80mg·kg-1)均可降低大鼠全脑缺血再灌注后升高的外周血白细胞和中性粒细胞计数、降低脑组织升高的皮质和海马MPO活性;改善脑组织皮质的缺血性改变。结论:黄芪提取物对大鼠全脑缺血再灌注损伤有明显的保护作用,其作用机制可能与其抗炎作用有关。AIM： To explore the protective effect of extract of astragalus （EA） on inflammatory reaction during global cerebral ischemia and reperfusion. METHODS： The model of global cerebral ischemia-reperfusion was established by four-vessel occlusion method. After global cerebral ischemia and reperfusion peripheral white blood cell and neutrophil were counted. The cortex and hippocampus myeloperoxidase （MPO） activities were determined by spectrophotometry. The pathological changes of cortex tissue were observed by light microscope. RESULTS： Compared with those in sham group, after global cerebral ischemia and reperfusion, the numbers of peripheral white blood cell and neutrophil and the activities of cortex and hippocampus MPO in control group were increased significantly. Compared with those in control group, the numbers of peripheral white blood cell and neutrophil of EA group were declined. Cortex and hippocampus MPO activities of EA group （40, and 80 mg·kg^-1） were lower than those in control group. The pathological changes of cortex tissue were less serious in rats treated with EA. CONCLUSION： EA has protective effect on cerebral ischemia and reperfusion injuries that may be relate to its anti-inflammatory effect.

关 键 词：黄芪提取物 缺血再灌注 白细胞 中性粒细胞 髓过氧化物酶 

分 类 号：R965[医药卫生—药理学]