淫羊藿总黄酮对去卵巢大鼠骨骼的影响  

Effects of epimedium pubescens flavonoids on skeleton of ovariectomized rats

作　　者：许碧连[1] 吴铁[1] 崔燎[1] 刘钰瑜[1] 邹丽宜[1] 

机构地区：[1]广东医学院药理教研室

出　　处：《中国临床药理学与治疗学》2005年第9期1059-1064,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：湛江市科技攻关项目及广东医学院面上科研项目(№XK0401)

摘　　要：目的:观察淫羊藿总黄酮(EF)对去卵巢大鼠骨骼的影响。方法:4.5月龄雌性SD大鼠40只,随机分为假手术组、去卵巢组、己烯雌酚(DES)组和淫羊藿总黄酮组。己烯雌酚组和淫羊藿总黄酮组大鼠行双侧卵巢去除术后,分别灌喂DES22.5μg·kg-1·d-1、EF300mg·kg-1·d-1,持续90d。用骨组织形态计量学等方法测量胫骨上段和第5腰椎松质骨及胫骨中段皮质骨的动态参数和静态参数,同时测量股骨钙、磷及羟脯氨酸含量,测量子宫湿重及子宫内膜厚度。结果:淫羊藿总黄酮可使去卵巢大鼠股骨钙、磷含量增加,胫骨上段的骨量有增加的趋势,但无统计学意义,腰椎和胫骨中段皮质骨的变化均不明显,子宫湿重及子宫内膜厚度也无明显变化。结论:淫羊藿总黄酮能有效预防去卵巢大鼠的股骨丢失,但不能有效预防胫骨上段和腰椎松质骨的丢失,对子宫没有刺激作用。AIM： To study the effects of epimedium pubescens flavonoids （EF） on the skeleton in ovariectomized rats. METHODS： Forty 4.5-month-old Sprague-Dawley female rats were randomly divided into 4 groups. Rats in sham group were sham-operated and treated by daily oral gavage with vehicle. Rats in other three groups were bilaterally ovariectomized （OVX） and treated by either daily oral gavage with vehicle, or diethylstilbestrol （DES） at 22.5 μg·kg^-1·d^-1, or EF at 300 mg·kg^-1· d^-1 for 90 days. Bone histomorphometric analysis of the proximal tibial metaphysis （PTM）, fifth lumbar vertebrae （LV5） and tibial shaft （Tx） was performed in undecalcified sections. The left femur was collected to determine bone weight, contents of calcium （Ca） , phosphorus （P） and hydroxyproline. The uterine weight and the uterine luminal epithelial thickness （ULET） were determined. RESULTS： A significant increase in contents of Ca and P of femur was found in EF group. A tendency of increase was found in % Tb. Ar of PTM, but no significant change was found in bone histomorphometric parameters of LV5 and Tx in EF group. EF had no effect on uterine weight and ULET. CONCLUSION： EF can prevent OVX-induced bone mineral loss of femur, but does not prevent bone loss of PTM and LV5.

关 键 词：淫羊藿总黄酮 骨质疏松 骨组织形态计量学 去卵巢大鼠 松质骨 皮质骨 

分 类 号：R284.1[医药卫生—中药学]