截形叶螨抗药性主导机制的研究  

STUDIES ON THE INSECTICIDAL RESISTANCE MECHANISMS OF COTTON SPIDER MITE

作　　者：范志金[1] 陈年春[2] 

机构地区：[1]四川师范大学,成都610066 [2]中国农业大学应用化学系

出　　处：《植物保护学报》1996年第2期175-180,共6页Journal of Plant Protection

摘　　要：研究室内汰选培育的截形叶螨(Tetranychus truncatus Ehara)抗性种群表明,截形叶螨抗久效磷的主导机制可能是乙酰胆碱酯酶(AchE)敏感性降低,谷胱甘肽—S—转移酶(GST)和多功能氧化酶(MFO)活性升高。抗三氟氯氰菊酯截形叶螨的主导机制是MFO和羧酸酯酶活性增强,以及羧酸酯酶发生变构,对底物(α—NA)的亲和力(R/S=5)和比活力(R/S=2)上升。生物测定结果TPP、SV_1和PBo对三氟氯氰菊酯都有增效作用,增效比值分别为2、3和6倍。截形叶螨抗三氯杀螨醇的主导机制是GST代谢毒物的能力增强,GST比活力上升(R/S=2),CH_3I对三氯杀螨醇有一定增效作用(2倍)。Sensitive and resistant populations of cotton spider mite (Tetranychus trunca-tus Ehara) obtained by laboratory selection were tested. The results indicated that the monocrotφphos-resistant mechanism in cotton spider mite (13 X ) was perhaps due to the decreased sentivity of acetylcholinesterase (AchE), increased activities of glutathione-S-transferase (GST) and mixed function oxidase (MFO). The resistant mechanism to cy-halothrin (182X) was due to the high activities of MFO and carboxylesterase, the structure of carboxylesterase was modified, it's affinity (R/S= 5) to the substrate (α-NA) and specific activities (R/S = 2) were apparently increased. TPP, SV1 and PBo had obvious synergism. It's synergistic value was 2-fold, 3-fold and 6-fold respectively. The resistance to dicofol (1313X) was mainly because of the high ability of detoxification of GST, the specific activity (R/S = 2) of GST increased and methyl iodide had synergism (2-fold) to dicofol.

关 键 词：截形叶螨 螨 种群 解毒酶 增效剂 抗药性 

分 类 号：S481.4[农业科学—农药学] S433.7[农业科学—植物保护]