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作 者:林治良[1,2] 王长春[1,2] 林瀚[1,2] 魏文麟[1,2]
机构地区:[1]福州市蔬菜科学研究所 [2]福建省农科院果树研究所
出 处:《福建农业大学学报》1996年第2期165-167,共3页Journal of Fujian Agricultural University
摘 要:在1/2MT(无机盐减半)附加0.5mgL-1BA和0.2mgL-1NAA的BM1培养基上建立试管株系.在MT附加0.5mgL-1BA和0.1mgL-1NAA的BM2快繁培养基上,每45d继代一次可获得平均达6.3倍的增殖率.继代芽苗接种在含0.2-1.The explants were induced to grow as established clonal on a BM 1 medium containing 1/2 MT(Salt half), supplemented with 0.5 mg·L -1 BA and 0.2 mg·L -1 NAA. Nodes were removed from the shoots and subcultured on BM 2 medium containing MT, supplemented with 0.5 mg·L -1 BA and 0.1 mg·L -1 NAA. An average of 6.3 nodes were produced from each explanted node after 45 days culture. Nodal cultures were transferred to a medium containing 0.2-1.0 mg·L -1 IBA to improve root initiation on the developing plantlets.
分 类 号:S668.203.5[农业科学—果树学]
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