牛卵丘－卵母细胞复合体体外成熟前后超微结构  

UITRASTRUCTURE OF CUMULUS-OOCYTE COMPLEX BEFORE AND AFTER IN WTRO MATURATION

作　　者：孙青原[1] 秦鹏春[1] 刘国艺[1] 杨庆章[1] 

机构地区：[1]东北农业大学生物工程系

出　　处：《东北农业大学学报》1996年第2期158-164,共7页Journal of Northeast Agricultural University

摘　　要：实验用透射电镜技术研究了牛卵立－卵母细胞复合体（ＣＯＣ）体外成熟前后卵母细胞、卵丘细胞及它们之间联系的结构特征．体外培养前，卵母细胞微绒毛数量较少，有的伸入秀明带中；各种细胞器集中分布于皮质区，形成“细胞器带”；核膜可打褶，核仁致密化．卵母细胞与卵丘细胞间及卵丘细胞之间形成间隙连接；卵丘细胞中线粒体致密、形态多样．成熟培养后．微绒毛增多．卵周隙形成；高尔基复合体消失，脂滴伴随着线粒体内迁．少数线粒体转变为幼稚型，皮质区形成“细胞器游离带’；皮质颗粒开始沿质膜下呈线状排列，但有的仍成团分布；排出第一极体处无微绒毛．中期赤道板附近无细胞器；卵丘细胞间及卵丘细胞与卵母细胞间联系中止；卵丘细胞中线粒体转变为幼稚型．The ultrastructural fcatures of oocyte,cumulus cells and their couplings of bovne cunmuLus-oocyte complexes(COC)before and after in vitro maturation were studied with transmission electron mieroscope. Before culture, the microvilli on oocyte surface were rare and some of them penetrated into the zona pellucida. Mitochondria, Golgi's complexes (Go), cortical granules(CGS),smooth endoplasmic reticulum(SER),annulate lamella and,lipid droplets gathered in the cortical region, forming an' organelle zone'. The membrane of nucleus which was the periphery of oocyte folded and the karyosphere was being formed.Membrane-bound veslcles distributed evenly. The eud of cumulus cell process expanded and penetrated into the oocyte cortical zone, forming gap jumctions with oocyte membrane. The adjacent cumulus cells madc functional conncctions by finger like interlock of mierovilli and small or large gap junctions.The electronic dense mitochondria in various shapes and rough cndoplasmic rcticulum were rich In cumulus cell cytoplasm.After culture,the number of oocyte microvilli increased and perivitelline space formed.Mitochondria, accompanied by lipid droplets scattered and moved centrally, some of which tfansformed into rare-cristaed ones and Go disappcared, forming a cortical' organelle-free zone' .The number of CGS increased and some of them distributed beneath the oolemma in a line manner, While others still gathered in Clusters in cortical region. Various kinds of organelles and inclusions cxisted in ooplasn and naked dense chromatin were found in the first polar body. The ooplasm ncar the metaphase plate was free of organelles and there were no microvilli on oocyte surface of this region.The couplings between oocyte and cumulus cells, and those between adjacent cumulus cells were interrupted. Many immature type mitochondria and lipid droplets appeared in cumulus cell cytoplasm.

关 键 词：牛 卵母细胞复合体 体外成熟 超微结构 

分 类 号：S823.1[农业科学—畜牧学] S852.1[农业科学—畜牧兽医]